| Study on ELISA for detection of sulfamethazine residues |
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| 作者姓名： | LI Jichang HE Liang XU Shiwen HUO Guicheng DING Liangjun LIU Ning |
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| 作者单位： | College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China |
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| 基金项目： | Key Items of National Technology Research Project，Heilongjiang Provincial Science and Technology Tackle Key Problem Project，Harbin Technical Fund |
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| 摘 要： | In this study, various factors of ELISA for detection of sulfamethazine residues were explored, the coating antigen was diluted to 1：400, the best coating condition was at 4℃ overnight, the working concentration of HRP-IgG enzyme conjugate was 1 ： 7 000. The pre-incubation time and incubation time was 30 min and 120 min, respectively, the substrate solution working time was 20 min. Two moL · L^-1 H2SO4 was used to stop the reaction and checked. A standard curve of direct competitive ELISA had been established to detect the sulfamethazine residues in milk. The detection limit of this method was 1.97 ng · mL^-1. The mean concentration of sulfamethazine required to inhibit 30% antibody was 7.1 ng · mL^-1. The linear range of the detection was 5-200 ng · mL^-1. The recovery ratio was between 73.20% and 91.16%. The CV% of within array and between arrays was less than 10%.
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| 关 键 词： | 磺胺甲嘧啶 单克隆抗体 药物残留检测 ELISA |
| 文章编号： | 1006-8104（2007）-03-0224-05 |
| 修稿时间： | 2006-09-26 |
Study on ELISA for detection of sulfamethazine residues |
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| LI Jichang HE Liang XU Shiwen HUO Guicheng DING Liangjun LIU Ning.Study on ELISA for detection of sulfamethazine residues[J].Journal of Northeast Agricultural University,2007,14(3):224-228. |
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| Authors: | LI Jichang HE Liang XU Shiwen HUO Guicheng DING Liangjun LIU Ning |
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| Abstract: | In this study, various factors of ELISA for detection of sulfamethazine residues were explored, the coating antigen was diluted to 1:400, the best coating condition was at 4℃ overnight, the working concentration of HRP-IgG enzyme conjugate was 1:7 000. The pre-incubation time and incubation time was 30 min and 120 min, respectively, the substrate solution working time was 20 min. Two moL· L-1 H2SO4 was used to stop the reaction and checked. A standard curve of direct competitive ELISA had been established to detect the sulfamethazine residues in milk. The detection limit of this method was 1.97 ng·mL-1. The mean concentration of sulfamethazine required to inhibit 30% antibody was 7.1 ng·mL-1. The linear range of the detection was 5-200 ng·mL-1. The recovery ratio was between 73.20% and 91.16%. The CV% of within array and between arrays was less than 10%. |
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| Keywords: | sulfamethazine monoclonal antibody ELISA residue detection |
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