| 鸡PDK4基因的组织表达分析 |
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| 引用本文： | 张榕婧,何小梅,闫景旭,韩琦琦,雷文龙,聂庆华,刘满清.鸡PDK4基因的组织表达分析[J].广东农业科学,2014,41(15):121-125. |
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| 作者姓名： | 张榕婧 何小梅 闫景旭 韩琦琦 雷文龙 聂庆华 刘满清 |
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| 作者单位： | 华南农业大学动物科学学院；华南农业大学兽医学院；广东省农业动物基因组学与分子育种重点实验室/农业部鸡遗传育种与繁殖重点实验室 |
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| 基金项目： | 国家自然科学基金（31172200）；国家“863”计划项目（2011AA100301，2013AA102501）；广东省战略性新兴产业核心技术攻关项目（2012A020800005） |
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| 摘 要： | 利用Real Time-PCR 的方法研究PDK4 基因在肉鸡中的组织表达规律尧在不同生长阶段的组织表达趋势及在不同生长速度肉鸡中的组织差异表达遥结果表明院渊1冤鸡PDK4 基因在肌肉组织渊包括腿肌尧胸肌尧心脏冤表达最高曰渊2冤肌肉组织PDK4 基因在12 胚龄的表达较低袁初生1 日龄时表达最高袁此后有所下降曰渊3冤在垂体尧胸肌尧心脏中袁PDK4 基因在杏花鸡中的表达高于白洛克鸡袁而在腿肌中的表达趋势相反曰胸肌中袁PDK4 基因表达在体重轻组中表达高于重组袁而在腿肌中的表达趋势相反遥
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| 关 键 词： | 肉鸡 PDK4基因 组织表达 |
Tissue expression of pyruvate dehydrogenase kinase 4 gene in broilers |
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| Abstract: | Using the method of Real Time-PCR, this study investigated the tissue expression pattern of pyruvate dehydrogenase kinase 4 (PDK4) gene in broilers, the tissue expression tendency in different growth stage of broilers and the differential tissue expression in broilers in different growth rate. The results showed that: (1) Chicken PDK4 gene was predominantly expressed in muscle tissues including breast muscle, leg muscle and heart. (2) The expression pattern of PDK4 gene in muscle tissues at different growth stages indicated that it was at a low level at 12 embryo age, and had thehighest primary body expression at 1 day, then it declined gradually. (3) In pituitary, breast muscle and heart, the expressions of PDK4 gene in Xinghua Chicken were higher than those in White Recessive Rock, which was reversed in leg muscle; in breast muscle, the expression of PDK4 gene in the light weight group was higher than that in high weight group,which was reversed in leg muscle. |
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| Keywords: | broilers PDK4 gene tissue expression |
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