| 兜兰茎腐病病原鉴定和生物学特性及室内药剂筛选研究 |
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| 引用本文： | 徐波,曾宋君,宋凤鸣,刘海涛,冯淑杰,张荣.兜兰茎腐病病原鉴定和生物学特性及室内药剂筛选研究[J].广东农业科学,2014,41(14):70-75. |
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| 作者姓名： | 徐波 曾宋君 宋凤鸣 刘海涛 冯淑杰 张荣 |
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| 作者单位： | 华南农业大学园艺学院；中国科学院华南植物园华南农业植物遗传育种重点实验室 |
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| 基金项目： | 广州市科技计划项目（Y2012J4300119） |
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| 摘 要： | 茎腐病是目前兜兰商品生产中最严重的一种病害，首次对广东兜兰栽培过程中的茎腐病进行比较深入的研究。通过形态学和分子生物学鉴定，确定导致兜兰茎腐病的病原菌为尖孢镰刀菌（Fusarium oxysporum），并对该菌的生物学特性进行初步研究。选用15种杀菌剂对该菌进行室内药效试验，其中多菌灵、苯醚甲环唑、肟菌酯、恶霉灵、普菌克、福窑福锌、妙丹和咪鲜胺8种药剂在使用浓度为500mg/L时，对该菌的菌丝抑制率和产孢抑制率均达100%，而且咪鲜胺对该菌的毒力最强、反应灵敏度最高，EC50值为0.08mg/L。
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| 关 键 词： | 兜兰 茎腐病 病原鉴定 生物学特性 杀菌剂筛选 |
Study on pathogen identification and biological characteristics of stem rot of Paphiopedilum and its indoor chemical control |
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| Abstract: | The stem rot is the most serious disease in the commodity production of Paphiopedilum. In this article, the stem rot of Paphiopedilum in Guangdong province has been firstly studied comprehensively. The result showed that the pathogen of stem rot of Paphiopedilum was identified as Fusarium oxysporum by morphologic observation and molecular identification. The preliminary research on biological characteristics of the pathogen and its sensitivity to 15 kinds of fungicides was also carried on. Under the concentrations 500 mg/L, Carbendazim, Difenoconazole, Trifloxystrobin,Hymexazol, Pujunke, Fufuxin, Miaodan and Prochloraz could effectively control mycelial growth and spore -bearing of pathogen of stem rot. Among the 8 fungicides, Prochloraz was the best and its EC50 was 0.08 mg/L. |
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| Keywords: | Paphiopedilum stem rot pathogen identification biological characteristics chemical control |
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