| 铜绿假单胞菌株CR56在黄瓜和番茄根围的定殖能力 |
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| 引用本文： | 楼兵干,张炳欣,Maarten Ryder.铜绿假单胞菌株CR56在黄瓜和番茄根围的定殖能力[J].浙江大学学报(农业与生命科学版),2001,27(2):183-186. |
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| 作者姓名： | 楼兵干 张炳欣 Maarten Ryder |
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| 作者单位： | 1. 浙江大学植物保护系，浙江杭州 310029
2. CSIRO Land and Water, Glen Osmonad, Southern Australian, 5064 |
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| 基金项目： | 澳大利亚国际农业发展中心资助项目（PN9680） |
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| 摘 要： | 铜绿假单胞菌株Pseudomonas aeruginosaCR56作为种子处理时，能有效地防治由腐霉Pythiumspp.和茄丝核菌Rhizoctonia solania引起的黄瓜和番茄的苗期猝倒病，利用该菌的抗利福平突变菌株CR56R9和lacZY标记菌株CR56RL4，研究其在黄瓜和番茄根围的定殖能力、种群动态和在根区的分布。研究表明，黄瓜根围比番茄根围更适合于该菌的定殖，其种群数量下降的幅度在番茄根围比在黄瓜根围更快。播后28d，菌株CR56R9和CR56RL4在黄瓜根围种群数量分别由7.63×107CFU/粒和4.88×107CFU/粒下降到9.85×106CFU/株和8.87×106CFU/株，在番茄根围则分别由3.0×107CFU/粒和2.13×107CFU/粒下降到4.48×105CFU/株和4.37×105CFU/株；该菌主要分布在猝倒病的发生部位——根基部，在根尖处检测不到该菌的存在。lacZY标记菌株CR56RL4与抗利福平突变菌株CR56R9，两者在黄瓜根围和番茄根围的存活能力基本一致。
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| 关 键 词： | Pseudomonas aeruginosa 根围 定殖 lacZY |
| 文章编号： | 1008-9209(2001)02-0183-04 |
| 修稿时间： | 2000年8月21日 |
Colonization of
Pseudomonas aeruginosa CR56 in the rhizosphere of cucumber and tomato |
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| LOU Bing gan ,ZHANG Bing xin ,Maarten Ryder.Colonization of
Pseudomonas aeruginosa CR56 in the rhizosphere of cucumber and tomato[J].Journal of Zhejiang University(Agriculture & Life Sciences),2001,27(2):183-186. |
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| Authors: | LOU Bing gan ZHANG Bing xin Maarten Ryder |
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| Institution: | LOU Bing gan 1,ZHANG Bing xin 1,Maarten Ryder 2 |
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| Abstract: | Pseudomonas aeruginosa strain CR56 is effective to controlcucumber and tomato seedlings damping-off disease caused by Pythium spp. and Rhizoctonia solania as a seed treatment. A strain resistant to rifampin, CR56R9, and a lacZY-marked derivative of wild strain CR56, CR56RL4, which were used to study the colonization ability, fluctuation of populations and distribution pattern on the rhizosphere of cucumber and tomato. The studies indicated the rhizosphere of cucumber was more suitable for the strains CR56R9 and CR56RL4 to colonize than the rhizosphere of tomato. The fluctuation of the two strains population was declined more quickly in the rhizosphere of tomato than that of cucumber. For 28 days after planting, the populations of CR56R9 and CR56RL4 in the rhizosphere of cucumber were declined from inoculum level 7.63×107and 4.88×107 CFU per seed to 9.85×106 and 8.87×106 CFU per plant，respectively, and the populations in the rhizosphere of tomato were declined from inoculum level 3.0×107 and 2.13×107 CFU per seed to 4.48×105 and 4.37×105 CFU per plant. The fluctuation of the introduced bacteria population was declined more quickly in the rhizosphere of tomato than of cucumber. It was found that the introduced bacteria were mainly in the location where damping-off disease occurred, namely in the base of roots. The introduced bacteria weren't detected in the tip of root. The living ability in the rhizosphere of cucumber and tomato between a lacZY gene marked strain and a rifampin-resistant strain was very similar. |
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| Keywords: | Pseudomonas aeruginosa rhizosphere colonization lacZY |
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