| 盐碱胁迫对柳树抗氧化酶的影响 |
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| 引用本文： | 刘铎,丛日春,高卫东,党宏忠,李庆梅,刘德玺,杨庆山.盐碱胁迫对柳树抗氧化酶的影响[J].水土保持通报,2017,37(5):53-57. |
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| 作者姓名： | 刘铎 丛日春 高卫东 党宏忠 李庆梅 刘德玺 杨庆山 |
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| 作者单位： | 1. 中国林业科学研究院荒漠化研究所,北京,100091;2. 中国林业科学研究院林业研究所国家林业局林木培育重点实验室,北京,100091;3. 山东省林科院,山东济南,250014 |
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| 基金项目： | 中央级公益性行业科研院所基本科研业务费“柳树抗复杂盐胁迫机理研究”（CAFYBB2016MA011）；江苏省科技支撑计划项目（BE2016328） |
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| 摘 要： | 目的]探讨柳树抗氧化系统对盐胁迫和碱胁迫响应的差异机制,以期为今后耐盐碱柳树选育工作提供科学依据。方法]以盐柳1号为试验材料,分别以中性盐NaCl和NaSO_4、碱性盐NaHCO_3和Na_2CO_3混合模拟盐、碱胁迫(两者摩尔比均为9∶1),共设计了5个梯度处理(0~200 mmol/L),共胁迫14d来研究盐与碱胁迫下柳树幼苗相关生理表现。结果]在碱胁迫情况下随着盐浓度升高,柳树叶片的SOD,POD,CAT这3种酶活性呈先升后降变化趋势,其中SOD在盐浓度为100mmol/L时达到最大为对照的1.48倍,POD和CAT在盐浓度为150mmol/L达到最大,分别为对照的4.26和5.24倍。中性盐胁迫下SOD和POD活性一直保持增大,在盐浓度为200mmol/L时达到最大值分别对照的1.33和3.26倍,在盐浓度为150mmol/L时CAT活性达到最大值为对照的2.79倍。碱胁迫下柳树叶片中丙二醛(MDA)含量明显高于中性盐胁迫下,当盐浓度为150mmol/L时为同浓度中性盐胁迫下的2.23倍。结论]从氧化胁迫角度分析,碱胁迫和盐胁迫是两种不同性质的胁迫,碱胁迫对柳树抗氧化系统造成的危害更大。
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| 关 键 词： | 柳树 酶活性 丙二醛 盐胁迫 碱胁迫 |
| 收稿时间： | 2014/8/8 0:00:00 |
| 修稿时间： | 2017/4/25 0:00:00 |
Effects of Salt and Alkali Stresses on Antioxidases of Willow |
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| LIU Duo,CONG Richun,GAO Weidong,DANG Hongzhong,LI Qingmei,LIU Dexi and YANG Qingshan.Effects of Salt and Alkali Stresses on Antioxidases of Willow[J].Bulletin of Soil and Water Conservation,2017,37(5):53-57. |
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| Authors: | LIU Duo CONG Richun GAO Weidong DANG Hongzhong LI Qingmei LIU Dexi and YANG Qingshan |
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| Institution: | Institute of Desertification Studies, Chinese Academy of Forestry, Beijing 100091, China,Institute of Desertification Studies, Chinese Academy of Forestry, Beijing 100091, China,Key Laboratory of Forest Silviculture of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China,Institute of Desertification Studies, Chinese Academy of Forestry, Beijing 100091, China,Key Laboratory of Forest Silviculture of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China,Shandong Forestry Research Institute, Jinan, Shandong 250014, China and Shandong Forestry Research Institute, Jinan, Shandong 250014, China |
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| Abstract: | Objective] To illustrate the comparative effects of salt and alkali stresses on antioxidases of willow, and to provide scientific basis for willow breeding.Methods] A willow cultivar Salix psammophila (Yanliu yihao) was used in this study. The stress conditions were simulated by five levels of salinities and alkalities with 14 days duration. The salinities were simulated by two neutral salts (NaCl and NaSO4) and alkalities were simulated by two alkaline salts (NaHCO3 and Na2CO3). The two neutral and alkaline salts were both mixed in the molar ratio of 9:1. The concentrations ranged from 0 to 200 mmol/L both for salt and alkali stress.Results] Under alkali stress, the activities of superoxide dismutase (SOD), peroxidase(POD) and CAT all presented a tendency of increasing at low concentration stress and then decreasing at high stress. The peak of SOD activity emerged when the willow was treated with 100 mmol/L, which was 1.48 times of the control''s; the peak of POD and CAT activities emerged when it was treated with 150 mmol/L, which were 4.26 and 2.79 times of the control''s values. Under alkali stress, the activities of SOD,POD and CAT all presented an increasing tendency. The peaks of SOD,POD and CAT activities all emerged at highest contrcentration of 200 mmol/L, they were 1.33,3.26 and 2.29 times of the control''s corresponding values. The content of MDA of willow leaf was higher in alkali treatment than that in salt treatment. When the concentrations of neutral and alkaline salts were at 150 mmol/L, the difference of MDA content was biggest.Conclusion] The alkali stress is different from salt stress, the alkali has stronger injury to willow than the salt stress has. |
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| Keywords: | willow enzyme activity MDA salt stress alkali stress |
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