The induction of tetraploidy in chicory (Cichorium intybus L. var. Magdebourg) by protoplast fusion |
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| Authors: | C Rambaud J Dubois J Vasseur |
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| Institution: | (1) Laboratoire de Physiologie Végétale, Université des Sciences et Techniques de Lille-Flandres Artois, 59655 Villeneuve d'Ascq, France |
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| Abstract: | Summary Fusions of chicory leaf protoplasts were realized in order to obtain a higher number of tetraploid plants compared to the traditional doubling techniques with colchicine. Thanks to the fusion technique, using a mixture of PEG, DMSO and a solution of CaCl2 (pH 10.5), 25% tetraploid (2n=36) plants were obtained. Among the 167 regenerated plants, only one aneuploid of a near tetraploid level (2n=33) was identified. |
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| Keywords: | chicory Cichorium intybus protoplast fusion tetraploids |
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