| PBI121/GPAT质粒的构建及对非洲菊转化* |
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| 引用本文： | 尹梅,程在全,夏小环,陈志伟,陈善娜.PBI121/GPAT质粒的构建及对非洲菊转化*[J].云南农业大学学报,2009,24(5):717-721. |
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| 作者姓名： | 尹梅 程在全 夏小环 陈志伟 陈善娜 |
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| 作者单位： | [1]云南大学生命科学学院生物技术系,云南昆明650091 [2]云南省农业科学院,云南昆明650205 |
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| 摘 要： | 为提高植物抗寒性，本文构建一个植物表达载体，并对非洲菊进行转化。用已克隆的强抗冷植物兵豆的甘油-3-磷酸转酰酶（GPAT）基因构建表达载体，将GPAT插入表达载体pBI121质粒取代该质粒上原有的GUS基因，并用PCR和酶切进行鉴定。用农杆菌介导的方法将该质粒转化到非洲菊中。结果表明该植物表达载体构建成功，同时对非洲菊高效转化体系进行探索，获得卡那霉素抗性苗7苗。
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| 关 键 词： | 甘油-3-磷酸转酰酶（GPAT） 构建 转化 非洲菊 |
| 收稿时间： | 2007-12-24 |
Construction and Transformation of Plasmid PBI121/GPAT into Gerbera hybrida |
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| YIN Mei,CHEN Zai-quan,XIA Xiao-huan,CHEN Zhi-wei,CHEN Shan-na.Construction and Transformation of Plasmid PBI121/GPAT into Gerbera hybrida[J].Journal of Yunnan Agricultural University,2009,24(5):717-721. |
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| Authors: | YIN Mei CHEN Zai-quan XIA Xiao-huan CHEN Zhi-wei CHEN Shan-na |
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| Institution: | 1. Department of Biotechnology,College of Live Science,Yunnan University,Kunming 650091,China；2. Yunnan Academy of Agricultural Sciences, Kunming 650223,China |
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| Abstract: | In order to enhance the chilling resistance of plant, a new plant expression vector was made and transformed into Gerbera hybrida. Glycerol 3 phosphate acyltransferase (GPAT) cDNA obtained from Lens culinaris was cloned into expression vector pBI121 by replacing GUS gene. The recombinant plasmid was confirmed by restriction endonuclease analysis and PCR detection. Agrobacterium method was used to transform the plasmid into Gerbera hybrida. The result shows that the new plant expression vector was constructed successfully. The genetic transformation system of Gerbera hybrida was studied and 7 kanamycin resistant plants were obtained. |
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| Keywords: | Glycerol 3 phosphate acyltransferase (GPAT) construction transformation Gerbera hybrida" target="_blank">Gerbera hybrida')" href="#">Gerbera hybrida |
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