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| 禽淋巴细胞性白血病的诊断与病毒亚群鉴定 | |
| 引用本文： | 罗明星,周碧君,李永明,汪德生,史开志,杨峰,赵航,袁圣,付鸣剑.禽淋巴细胞性白血病的诊断与病毒亚群鉴定[J].中国动物检疫,2009,26(3):35-37. |
| 作者姓名： | 罗明星 周碧君 李永明 汪德生 史开志 杨峰 赵航 袁圣 付鸣剑 |
| 作者单位： | 1. 贵州大学动物科学院,贵州贵阳,550025 2. 贵州大学动物疫病研究所,贵州贵阳,550025 3. 贵阳市农业局兽防站,贵州贵阳,550000 |
| 基金项目： | 贵阳市农业局科学技术计划项目项目，贵阳市农业局科学技术计划，贵州省农业厅兽医科技计划 |
| 摘 要： | 采用病理组织学方法对某商品蛋鸡场送检的发病鸡进行实验室诊断,在肿胀的肝、脾、肾和法氏囊组织切片中观察到成淋巴细胞增生病变。使用禽白血病A、B亚群抗体检测试剂盒对血清样本进行间接ELISA试验,抗体阳性率达到44.4%(12/27)。采集12只病死鸡的肝脏材料提取DNA样本,11份样本中扩增出ALV-A亚群特异性的病原核酸(691bp),检出率达到91.6%(11/12)。将扩增的目的基因克隆与测序,截取gp85基因片段可变区序列与ALV-A、B、C、D和E亚群参考毒株的相应序列进行比较,同源性分别达到89.0-89.6%、67.1-67.7%、69.1-70.1%、69.1-69.5%和70.9-72.0%。结果证实本次疫病是由ALV-A亚群病毒引起的淋巴细胞性白血病。 |
| 关 键 词： | 禽白血病病毒 A亚群 血清学检测 病理组织学检查 PCR鉴定、淋巴细胞性白血病 |
Diagnosis of avian lymphoid Leukosis and identification of virus subgroup |
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| Luo mingxing,Zhou bijun,Li yongming,Wang desheng,Shi kaizhi,Yang feng,Zhao hang,Yuan sheng,Fu mingjian.Diagnosis of avian lymphoid Leukosis and identification of virus subgroup[J].China Journal Of Animal Quarantine,2009,26(3):35-37. | |
| Authors: | Luo mingxing Zhou bijun Li yongming Wang desheng Shi kaizhi Yang feng Zhao hang Yuan sheng Fu mingjian |
| Institution: | 1 (1.College of Animal Science,Guizhou University,Guizhou Guiyang 550025;2.Insitute of Animal Disease,Guizhou university,Guiyang 550025,China; 3.Agricultural bureau Veterinary station,Guiyang,550000,China)) |
| Abstract: | Histopathological method was used for the diagnosis the diseased chickens in a commercial layer farm. The lymphoblast proliferative lesions were observed in tissue slides of swollen liver,spleen, kidney and Fabricius bursa.The serum samples were detected using avian leukosis virus antibody test kit-subgroup A,B (ALV-AB) and the antibody positive rate reached 44.4%(12/27). The total DNA samples were extracted from liver materials of 12 dead chickens. A 691 bp fragment was amplified from 11 DNA samples,which was specific for the subgroup A of ALV and the detective rate accounted for 91.6% (11/12).The amplified target genes were cloned and sequenced. The comparison was made against the variable region of gp85 gene with subgroup A、B、C、D and E of ALV. The homology reached 89.0～89.6% 、67.1～67.7% 、69.1～70.1% 、69.1～69.5% 和 70.9～72.0% respectively. It was demonstrated that the lymploid leucosis was caused by subgroup A of ALV. |
| Keywords: | Avian leukosis virus Subgroup A Seratogical detection Histopathalogical examination PCR identification Lymphoid leukosis |
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