Somatic embryogenesis and plant regeneration in Opuntia ficus-indica (L.) Mill. (Cactaceae) |
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Authors: | Francisco Linhares Arruda Ferreira Gomes Fabíola Fernandes Heredia Priscilla Barbeta e Silva Olivardo Facó Francisco de Assis de Paiva Campos |
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Institution: | 1. Universidade Estadual do Ceará, Centro de Ciências da Saúde, Av. Parajana 1700, CEP 60 740-000, Fortaleza, CE, Brazil;2. Universidade Federal do Ceará, Departamento de Bioquímica e Biologia Molecular, Caixa Postal 6039, CEP 60455-900, Fortaleza, CE, Brazil |
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Abstract: | We present here a method for the regeneration of the prickly-pear (Opuntia ficus-indica (L.) Mill.) through somatic embryogenesis (SE). Direct SE was induced by cultivating shoot apices devoid of leaf primordia on semisolid Murashige and Skoog (MS) basal medium supplemented with 4-amino 3,5,6-trichloropicolinic acid (picloram) at 4 mg l−1. Somatic embryogenesis was influenced by the type, age, physiological and developmental stage of the explants, and also by light conditions, wounding, and the sucrose concentration in the induction medium. A histological analysis confirmed SE by revealing the presence of a closed vascular system in the developing embryos and the absence of a vascular connection between the somatic embryos and the explant. |
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Keywords: | Somatic embryogenesis Prickly-pear Opuntia ficus-indica Picloram |
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