| 水稻第6染色体短臂谷壳硅含量QTL的分解 |
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| 引用本文： | 吴季荣,范方军,杜景红,樊叶杨,庄杰云.水稻第6染色体短臂谷壳硅含量QTL的分解[J].中国水稻科学,2010,24(1):12-17. |
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| 作者姓名： | 吴季荣 范方军 杜景红 樊叶杨 庄杰云 |
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| 作者单位： | 中国水稻研究所,国家水稻改良中心/水稻生物学国家重点实验室,浙江,杭州,310006 |
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| 基金项目： | 国家自然科学基金资助项目，国家863计划资助项目，农业部超级稻专项资助项目 |
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| 摘 要： | 报道了水稻谷壳硅含量QTLqHUS6的分解研究。针对前期定位的qHUS-6区域,应用在第6染色体短臂RM587-RM19784区间分离、背景基本纯合的F2:3群体,将qHUS-6分解为2个QTL;其中,qHUS-6-1位于目标区间的上部,qHUS-6-2位于下部。同时,筛选出在目标区间内携带更小杂合片段的3个单株。其中,2株覆盖qHUS-6-1区间,各自交产生了1个F2:3群体,应用这2个群体将qHUS-6-1定位于RM510和RM19417之间约147.0kb的区间内;另1株覆盖qHUS-6-2区间,自交产生了1个F2:3群体,从中挑选出在qHUS-6-2区间具有不同基因型组成的5套F3株系,将qHUS-6-2分解为qHUS-6-2a和qHUS-6-2b,分别位于RM19706-RM19795和RM314-RM19665区间。
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| 关 键 词： | 水稻 数量性状基因座 剩余杂合体 谷壳硅含量 |
| 收稿时间： | 1900-01-01; |
Dissection of QTLs for Hull Silicon Content on the Short Arm of Rice Chromosome 6 |
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| WU Ji-rong,FAN Fang-jun,DU Jing-hong,FAN Ye-yang,ZHUANG Jie-yun.Dissection of QTLs for Hull Silicon Content on the Short Arm of Rice Chromosome 6[J].Chinese Journal of Rice Science,2010,24(1):12-17. |
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| Authors: | WU Ji-rong FAN Fang-jun DU Jing-hong FAN Ye-yang ZHUANG Jie-yun |
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| Institution: | WU Ji-rong,FAN Fang-jun,DU Jing-hong,FAN Ye-yang,ZHUANG Jie-yun(Chinese National Center for Rice Improvement/State Key Laboratory of Rice Biology,China National Rice Research Institute,Hangzhou 310006,China) |
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| Abstract: | The dissection of the QTL qHUS-6 for hull silicon content in rice which was previously located on the short arm of rice chromosome 6 is reported. Using an F2:3 population segregated in the RM587-RM19784 region harboring qHUS-6 in an isogenic background,two QTLs for hull silicon content were detected,of which qHUS-6-1 was located in the upper region and qHUS-6-2 in the lower region. Three plants carrying smaller heterozygous segments in the target region were selected,of which two covered the qHUS-6-1 region... |
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| Keywords: | rice (Oryza sativa) quantitative trait locus residual heterozygous line hull silicon content gene mapping |
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