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| 新城疫病毒感染绵羊诱导免疫应答反应的实验研究 | |
| 引用本文： | 王善辉,池贤凤,李安,高三阳,李书光,王文秀,沈志强.新城疫病毒感染绵羊诱导免疫应答反应的实验研究[J].中国预防兽医学报,2011,33(10). |
| 作者姓名： | 王善辉 池贤凤 李安 高三阳 李书光 王文秀 沈志强 |
| 作者单位： | 1. 山东省滨州畜牧兽医研究院重点实验室,山东滨州,256600 2. 山东绿都生物科技有限公司,山东滨州,256600 3. 山东省滨州畜牧兽医研究院重点实验室,山东滨州256600;山东绿都生物科技有限公司,山东滨州256600 |
| 基金项目： | 山东省自主创新成果转化重大专项计划(2008ZHZX1A1103) |
| 摘 要： | 为探索新城疫病毒(NDV)作为羊病毒病疫苗载体的可行性,验证NDV感染绵羊的可能性,本研究采用NDV Clone-30株分别通过滴鼻和气管灌注两种途径接种绵羊,接种后观察临床症状,检测接种动物排毒情况,通过血凝抑制试验检测接种绵羊特异性血凝抑制抗体,ELISA试验检测接种绵羊血清特异性IgG抗体,微量中和试验检测接种绵羊血清中和抗体.研究结果表明,NDV在绵羊体内是非致病性的,并且通过两种接种途径在绵羊体内均可以产生血凝抑制抗体、NDV特异性IgG抗体和中和抗体反应.本研究结果表明NDV有可能作为宿主限制性疫苗载体用于预防无特效疫苗的羊类疾病,且与气管灌注接种途径比,滴鼻接种途径相对安全. |
| 关 键 词： | 新城疫病毒 免疫应答 绵羊 抗体 |
Experimental infection of sheeps with Newcastle disease virus induces immune response |
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| WANG Shan-hui,CHI Xian-feng,LI An,GAO San-yang,LI Shu-Guang,WANG Wen-xiu,SHEN Zhi-qiang.Experimental infection of sheeps with Newcastle disease virus induces immune response[J].Chinese Journal of Preventive Veterinary Medicine,2011,33(10). | |
| Authors: | WANG Shan-hui CHI Xian-feng LI An GAO San-yang LI Shu-Guang WANG Wen-xiu SHEN Zhi-qiang |
| Institution: | WANG Shan-hui 1,CHI Xian-feng 2,LI An 2,GAO San-yang 1,LI Shu-Guang 1,WANG Wen-xiu 1,SHEN Zhi-qiang 1,2 (1.Shandong Binzhou Animal Science & Veterinary Medicine Institute,Binzhou 256600,China,2.Shandong Lvdu Bio-Sciences & Technology Co.Ltd.,China) |
| Abstract: | To test the possibility of Newcastle disease virus(NDV) as NDV associated vaccine vactor in sheep,two groups of sheep were inoculated intranasally and intratracheally with NDV-30 strain,respectively,and the clinical signs and viral replication were evaluated.for detection of anti-HN antibodies,neutralizing antibodies and NDV-specific IgG antibodies in serum were dectected by HI test,microneutralization test and ELISA test,respectively.The results showed that NDV-30 was attenuated in this unnatural host.Anti... |
| Keywords: | Newcastle disease virus immune response sheep antibodies |
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