| 牛源犬新孢子虫MAG1基因的克隆及在Vero细胞中表达 |
| |
| 引用本文： | 焦石,贾立军,刘明明,黄国明,张守发.牛源犬新孢子虫MAG1基因的克隆及在Vero细胞中表达[J].中国预防兽医学报,2011,33(11). |
| |
| 作者姓名： | 焦石 贾立军 刘明明 黄国明 张守发 |
| |
| 作者单位： | 延边大学农学院动物医学系,吉林延吉,133002 |
| |
| 基金项目： | 吉林省自然科学基金项目 |
| |
| 摘 要： | 为了解牛源犬新孢子虫MAG1基因的生物学特性,本实验应用PCR技术扩增牛源犬新孢子虫MAG1基因,构建真核表达重组质粒pVAX-MAG1,将鉴定正确的pVAX-MAG1重组质粒转染Vero细胞,应用间接荧光检测方法(1FA)和western blot技术检测MAG1基因在Vero细胞中的表达.结果显示,扩增的牛源犬新孢子虫MAG1基因长度为1047bp,与GenBank中登录的MAG1 (EF580924.1)核苷酸序列同源性为99％,IFA检测MAG1基因在Vero细胞中获得瞬时表达,western blot分析表达蛋白的分子量为39 ku,具有较好的反应原性.本实验为新孢子虫病核酸疫苗与诊断试剂盒的研究奠定了基础.
|
| 关 键 词： | 犬新孢子虫 MAG1基因 真核表达 |
Eukaryotic expression of bovine Neospora caninumMAG1 gene in Vero cells |
| |
| JIAO Shi,JIA Li-jun,LIU Ming-ming,HUANG Guo-ming,ZHANG Shou-fa.Eukaryotic expression of bovine Neospora caninumMAG1 gene in Vero cells[J].Chinese Journal of Preventive Veterinary Medicine,2011,33(11). |
| |
| Authors: | JIAO Shi JIA Li-jun LIU Ming-ming HUANG Guo-ming ZHANG Shou-fa |
| |
| Institution: | JIAO Shi,JIA Li-jun,LIU Ming-ming,HUANG Guo-ming,ZHANG Shou-fa(Department of Veterinary Medicine,Agricultural College of Yanbian University,Yanji 133002,China) |
| |
| Abstract: | To inestigate the biological characteristics of bovine Neospora caninum MAG1 gene,the gene was amplified by PCR and inserted into eukaryotic expression vector.The resultant recombinant plasmid of pVAX-MAG1 was transfected into Vero cells and the expression of MAG1 was detected by indirect immunofluorescent assay,and western blot showed that the molecular weight of the protein was 39 ku and the protein had a positve reaction with anti MAG1 serum.The results could be useful to DNA vaccine development. |
| |
| Keywords: | Neospora caninum MAG1 gene eukaryotic expression |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
