|
|||||
|
|
| 荧光定量RT-PCR方法与兔体定型热试验对于检测猪瘟兔化弱毒疫苗的平行关系 | |
| 引用本文： | 葛叶,张兴娟,朱庆虎,韩秋影,王牟平,李伟杰,孙建宏,仇华吉.荧光定量RT-PCR方法与兔体定型热试验对于检测猪瘟兔化弱毒疫苗的平行关系[J].中国预防兽医学报,2011,33(9). |
| 作者姓名： | 葛叶 张兴娟 朱庆虎 韩秋影 王牟平 李伟杰 孙建宏 仇华吉 |
| 作者单位： | 1. 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/猪传染病研究室,黑龙江哈尔滨150001;中国农业科学院哈尔滨兽医研究所维科生物技术开发公司,黑龙江哈尔滨150069 2. 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/猪传染病研究室,黑龙江哈尔滨,150001 3. 中国农业科学院哈尔滨兽医研究所维科生物技术开发公司,黑龙江哈尔滨,150069 |
| 基金项目： | 国家科技支撑计划(2006BAD06A03) |
| 摘 要： | 为探讨荧光定量RT-PCR技术与传统兔体定型热试验对于检测猪瘟兔化弱毒疫苗的平行关系,本研究采用荧光定量RT-PCR方法与兔体定型热试验对5份猪瘟兔化弱毒细胞苗成品和6份半成品样品进行平行检测.结果表明,两种检测方法存在正相关性,最低105个病毒拷贝可以使家兔产生定型热反应,最低103个病毒拷贝可以使家兔产生轻热反应.荧光定量RT-PCR检测方法的检测过程仅需3.5h,大大缩短了猪瘟疫苗的检测时间,该方法可以补充传统的兔体定型热反应用于猪瘟兔化弱毒疫苗半成品与成品的检验. |
| 关 键 词： | 猪瘟兔化弱毒疫苗 荧光定量RT-PCR 免体定型热试验 |
Parallel relationship between real-time RT-PCR and fever reaction method in rabbits for testing hog cholera lapinized virus vaccines |
|
| GE Ye,ZHANG Xing-juan,ZHU Qing-hu,HAN Qiu-ying,WANG Mu-ping,LI Wei-Jie,SUN Jian-hong,QIU Hua-ji.Parallel relationship between real-time RT-PCR and fever reaction method in rabbits for testing hog cholera lapinized virus vaccines[J].Chinese Journal of Preventive Veterinary Medicine,2011,33(9). | |
| Authors: | GE Ye ZHANG Xing-juan ZHU Qing-hu HAN Qiu-ying WANG Mu-ping LI Wei-Jie SUN Jian-hong QIU Hua-ji |
| Institution: | GE Ye1,2,ZHANG Xing-juan1,ZHU Qing-hu2,HAN Qiu-ying1,WANG Mu-ping2,LI Wei-Jie2,SUN Jian-hong2,QIU Hua-ji1(1.Division of Swine Infectious Diseases,State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China,2.Harbin Weike Biotechnology Development Company,Harbin 150069,China) |
| Abstract: | To evaluate a parallel relationship between the real-time RT-PCR method and fever reaction method in rabbits,we detected 5 finished and 6 semi-finished products of hog cholera lapinized virus(HCLV) vaccines.The results showed that both methods had positive correlation,105 copies of HCLV were able to induce typical fever reaction and 103 copies induce moderate fever reaction in rabbits.Only 3.5 hours was needed for the real-time RT-PCR,indicating that the real-time RT-PCR could be an alternative to tradition... |
| Keywords: | hog cholera lapinized virus real-time RT-PCR fever reaction method in rabbits |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |