| 应用ViewRNA技术特异性检测感染细胞中的不同马传染性贫血病毒株 |
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| 引用本文： | 王珊珊,马建,史楠,刘强,韦华冕,周建华.应用ViewRNA技术特异性检测感染细胞中的不同马传染性贫血病毒株[J].中国预防兽医学报,2011,33(10). |
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| 作者姓名： | 王珊珊 马建 史楠 刘强 韦华冕 周建华 |
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| 作者单位： | 1. 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/大动物传染病研究室,黑龙江哈尔滨,150001
2. 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/大动物传染病研究室,黑龙江哈尔滨150001;东北林业大学野生动物资源学院,黑龙江哈尔滨150040 |
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| 基金项目： | 国家自然科学基金青年基金(31001049); 十一五重大传染病专项(2008ZX10001-1010); 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室开放基金(SKLVBD201001); 东北林业大学青年拔尖人才支持计划(YTTP-1011-02) |
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| 摘 要： | 为了对体外培养细胞感染的不同马传染性贫血病毒(EIAV)株进行特异的鉴别检测,本研究基于一种新的RNA原位杂交-ViewRNA技术,通过设计合成针对EIAVFDDV13和EIAVUK3病毒株基因组RNA的特异性探针,并结合信号放大技术和荧光标记技术,同时通过激光共聚焦显微镜进行观察,建立了可时两种不同株的EIAV基因组RNA进行细胞内原位检测的方法.研究结果表明,两组探针能够特异地与感染细胞中EIAVFDD13和EIAVUK3的病毒RNA相结合,可在两种不同EIAV株共感染的宿主细胞中实现对两种毒株的有效鉴别和定位检测.该方法为进一步研究EIAV与宿主细胞间相互作用提供了有效手段.
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| 关 键 词： | 马传染性贫血病毒 原位杂交 ViewRNA技术 |
In situ detection of co-infected equine infectious anemia virus heterologous strains in fetal donkey dermal cells using a ViewRNA assay |
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| WANG Shan-shan,MA Jian,SHI Nan,LIU Qiang,WEI Hua-mian,ZHOU Jian-hua.In situ detection of co-infected equine infectious anemia virus heterologous strains in fetal donkey dermal cells using a ViewRNA assay[J].Chinese Journal of Preventive Veterinary Medicine,2011,33(10). |
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| Authors: | WANG Shan-shan MA Jian SHI Nan LIU Qiang WEI Hua-mian ZHOU Jian-hua |
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| Institution: | WANG Shan-shan 1,MA Jian 1,2,SHI Nan 1,LIU Qiang 1,WEI Hua-mian 1,ZHOU Jian-hua 1 (1.Division of Livestock Infectious Diseases,State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China,2.College of Wildlife Resource,Northeast Forestry University,Harbin 150040,China) |
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| Abstract: | The ViewRNA assay is a novel RNA in situ hybridization method with the high sensitivity to detect even a single RNA copy for specifically differential detection and location of different virus strains in co-infected cell.To detect 2 different strains of equine infectious anemia virus(EIAV) in infected cells,two nucleotide probes were designed and synthesized,of which were capable to hybridize with the genomic RNA of different EIAV strains.The EIAV FDDV13 and EIAV UK3 were co-infected the fetal donkey dermal... |
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| Keywords: | equine infectious anemia virus in situ hybridization ViewRNA technology |
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