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| 水双相法分离泡桐幼苗根细胞质膜的研究 | |
| 引用本文： | 洪剑明,贾慧君,郑槐明.水双相法分离泡桐幼苗根细胞质膜的研究[J].林业科学,2001,37(1):23-27. |
| 作者姓名： | 洪剑明 贾慧君 郑槐明 |
| 作者单位： | 1. 首都师范大学生物系, 2. 中国林业科学研究院, 3. 中国林学会, |
| 基金项目： | 国家自然科学基金资助项目!稳态营养苗木根细胞质膜的动态研究 (39770 6 14 ) |
| 摘 要： | 通过对草本植物水双相法分离细胞质膜技术的改进，建立起木本植物泡桐根细胞质膜的分离纯化方法。其改进主要有：在匀浆介质中添加了抗坏血酸，加大了聚乙烯聚吡咯烷酮的用量，由草本植物一般用量０．０５％－０．１％增至０．６％；对第１次双相分离后下相液中剩余的质膜进行了再回收。通过对质膜和细胞器各项标记酶指标的测定，确定了６．３％，葡聚糖（Dextran)T-500和６．３％的聚乙二醇（ＰＥＧ）４０００为主组成的两相系统分离的根细胞质膜具有较高的收率和纯度。该方法的建立为细胞和分子水平上研究木根系对离子吸收机理与生长发育的关系打下了基础。 |
| 关 键 词： | 泡桐 水双相分离法 细胞质膜 标记酶 氧化还原活性 幼苗 根部 |
| 修稿时间： | 2000年3月16日 |
STUDIES ON SEPARATING PLASMA MEMBRANE FROM ROOTS OF PAULOWNIA SEEDLING BY AQUEOUS TWO-PHASE PARTITIONING |
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| Hong Jianming,Jia Huijun,Zheng Huaiming.STUDIES ON SEPARATING PLASMA MEMBRANE FROM ROOTS OF PAULOWNIA SEEDLING BY AQUEOUS TWO-PHASE PARTITIONING[J].Scientia Silvae Sinicae,2001,37(1):23-27. | |
| Authors: | Hong Jianming Jia Huijun Zheng Huaiming |
| Abstract: | This paper reports the method of partitioning and purifying plasma membrane from roots of woody plants of Paulownia by improving the method of preparing pla sma membrane by aqueous two-phase partitioning in herbaceous plants. The main i mprovements are adding ascorbic acid and increasing an amount of insoluble pvpp( polyvinylpolypyrrolidone) from 0.05%～0.1% of herbaceous plants to 0.6% in th is homogenization medium, and then again collecting the plasma membrane from the lo wer phase after the first aqueous two-phase partitioning. Through determining t he different kinds of marker enzymes of plasma membranes and organelles, that pl asma membrane preparations of high yield and purity were easily obtained in aque ous polymer two-phase systems mainly constituted by 6.3% Dextran T-500 and 6.3 % polyethylene glycol 4000. This method lays the foundations for the studies on the relation between the principles of roots absorption ions and growth, develop ment of a woody plant at cellular and molecular level. |
| Keywords: | Paulownia Aqueous two\|phase partitioning Plasma membrane Marker enzyme Activity of oxidation\|reduction |
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