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壳聚糖对棉花种子萌发及相关酶活性的影响
引用本文:宋淑淑,朱启忠,王蓉,闫杰,孔晓静.壳聚糖对棉花种子萌发及相关酶活性的影响[J].种子,2012,31(4):40-42.
作者姓名:宋淑淑  朱启忠  王蓉  闫杰  孔晓静
作者单位:山东大学(威海)海洋学院,山东威海,264209
基金项目:山东大学(威海)科研立项资助项目(编号:A 11066)
摘    要:用0.1%、0.2%、0.3%、0.4%、0.5%五种浓度的壳聚糖溶液处理棉花种子12h,以蒸馏水浸种12h为对照.室温培养7d,每天自然光照8h,棉花种子的发芽率、发芽势、萌发指数、活力指数及α-淀粉酶活性、过氧化物酶活性和过氧化氢酶活性均比对照高,且随着壳聚糖浓度的增大各指标均呈先升高后降低的趋势,处理浓度为0.2%时,种子的发芽率、发芽势、萌发指数、活力指数及α-淀粉酶活性、过氧化物酶活性和过氧化氢酶活性分别提高了16.7%、3.17%、2.63、30.7、96.87%、37.42%、33.78%,效果最佳.

关 键 词:壳聚糖  棉花  种子萌发  酶活性

Effect of Chitosan on Seed Germination and Relevant Enzymes,Activity of Cotton
SONG Shu-shu , ZHU Qi-zhong , WANG Rong , YAN Jie , KONG Xiao-jing.Effect of Chitosan on Seed Germination and Relevant Enzymes,Activity of Cotton[J].Seed,2012,31(4):40-42.
Authors:SONG Shu-shu  ZHU Qi-zhong  WANG Rong  YAN Jie  KONG Xiao-jing
Institution:(College of Ocean,Shandong University(Weihai),Weihai Shandong 264209,China)
Abstract:Cotton seeds were soaked in chitosan solution of different concentrations(0.1%,0.2%,0.3%,0.4%,0.5%)for 12 hours in comparison with seeds in water.Then cultured them for 7 days at room temperature and 8 hours at natural light every day.Seed germination rate,germination power,germination index,vigor index and activity of a-amylase,POD and CAT were higher than the control.With the increasing of chitosan concentration,all indexes increaded at first and then decreased,When the concentration was 0.2%,the seed germination rate,germination energy,germination index,vigor index,a-amylase,POD and CAT activity were increased by 16.7%,3.17%,2.63,30.7,96.87%,37.42% and 33.78% respectively,and it was the optimum concentration.
Keywords:chitosan  cotton  seed germination  enzymes’activity
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