| 硒浸种对豌豆种子萌发的生理生化效应 |
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| 引用本文： | 何士敏,张燕,易涵.硒浸种对豌豆种子萌发的生理生化效应[J].种子,2012,31(2):42-47. |
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| 作者姓名： | 何士敏 张燕 易涵 |
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| 作者单位： | 长江师范学院生命科学与技术学院,重庆涪陵,408100 |
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| 摘 要： | 以豌豆(Pisum sativum Linn)种子为研究材料,设置了6个硒浸种浓度(0、3、5、10、15、20 mg/L),测定了硒浸种的豌豆种子萌发期电解质外渗率和种子发芽情况,测定了CAT、POD、SOD、PPO酶活性,旨在探讨提高种子活力的途径.结果表明:适宜浓度硒浸种可以提高豌豆种子的活力、电解质外渗率和相关酶的活性.3 mg/L硒溶液处理提高种子SOD活性;5 mg/L硒溶液处理明显提高种子发芽率、活力指数和CAT、PPO活性;10 mg/L硒溶液可提高种子POD活性.
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| 关 键 词： | 豌豆 硒 活力指数 电解质外渗率 酶活性 |
Effect of Soaking Seed with Silenium on Physiological and Biochemical Characters of Pisum sativum Germinating |
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| HE Shi-Min
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ZHANG Yan
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YI Han.Effect of Soaking Seed with Silenium on Physiological and Biochemical Characters of Pisum sativum Germinating[J].Seed,2012,31(2):42-47. |
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| Authors: | HE Shi-Min ZHANG Yan YI Han |
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| Institution: | (Department of Life Science and Technology,Chang jiang Teacher’s College,Fuling Chongqing 408003,China) |
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| Abstract: | The study dealt with the effect of Selenium on germination of Pisum sativum Linn seeds in different concentrations(0,3.0,5.0,10.0,15.0 and 20.0 mg/L).And tested catalase(CAT) and peroxidase(POD) and polyphenol oxidase(PPO) and superoxide dismutase(SOD)activity of soybean seeds soaked with Se through colorimetric and oxidize-self method.At the same time,tested electrolyte oozing rate and seeds germinating situation,the peroxidase activity,the superoxide dismutase activity and the polyphenol oxidase activity.The results demonstrated that the superoxide dismutase(SOD) activity reached the highest point at the treatment of 3 mg/L Selenium,soaked seeds with Selenium could increase seeds vigor and relative enzymatic activity obviously.The treatment of 5 mg/L Selenium could increase germinating rate and vigor index and CAT,PPO activity.The superoxide peroxydase(POD) activity reached the highest point at the treatment of 3 mg/L Selenium. |
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| Keywords: | Pisum sativum L selenium vigor index electrolyte oozing rate enzymatic activity |
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