| 生物与非生物胁迫下水稻CIPK基因的鉴定分析 |
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| 引用本文： | 陈析丰,顾志敏,刘峰,马伯军,张红生.生物与非生物胁迫下水稻CIPK基因的鉴定分析[J].中国水稻科学,2010,24(6). |
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| 作者姓名： | 陈析丰 顾志敏 刘峰 马伯军 张红生 |
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| 作者单位： | 南京农业大学作物遗传与种质创新国家重点实验室;浙江师范大学化学与生命科学学院; |
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| 基金项目： | 国家自然科学基金，浙江省自然科学基金，国家转基因生物新品种培育重大科技专项资助项目，浙江师范大学创新团队项目 |
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| 摘 要： | 类钙调磷酸酶B亚基蛋白(calcineurin B-like proteins,CBL)是植物细胞中重要的Ca2+传感器,必须与蛋白激酶CIPK(CBL-interacting protein kinases)特异性互作才能发挥生物学功能.根据水稻OsCIPK(Oryza sativa CIPK)基因家族的预测序列,从粳稻日本晴(Nipponbare)中克隆了15个OsCIPK基因.基因结构分析表明15个OsCIPK可归为多内含子和少内含子两类,OsCIPK3和OsCIPK24的mRNA存在可变剪接.系统进化树分析显示水稻CIPK家族与拟南芥、杨树来源于同一个祖先.这15个OsCIPK在不同程度上受生物胁迫(白叶枯病)和非生物胁迫(Hg2+、高盐、冷和ABA)的诱导表达;其中,5个基因(OsCIPK1、OsCIPK2、OsCIPK10、OsCIPK11和OsCIPK12)在接种白叶枯病菌后表达显著上调;而4个基因(OsCIPK2、OsCIPK10、OsCIPK11和OsCIPK14)的表达受所有胁迫处理诱导,表明这些基因可能参与多种逆境信号的转导.揭示了CIPK可能在植物的生物和非生物胁迫应答中都具有重要的作用.
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| 关 键 词： | 水稻 CIPK家族 基因表达 胁迫 白叶枯病 |
Molecular Analysis of Rice CIPKs Involved in Biotic and Abiotic Stress Responses |
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| CHEN Xi-feng,GU Zhi-min,LIU Feng,MA Bo-jun,ZHANG Hong-sheng.Molecular Analysis of Rice CIPKs Involved in Biotic and Abiotic Stress Responses[J].Chinese Journal of Rice Science,2010,24(6). |
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| Authors: | CHEN Xi-feng GU Zhi-min LIU Feng MA Bo-jun ZHANG Hong-sheng |
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| Institution: | CHEN Xi-feng1,2,GU Zhi-min2,LIU Feng2,MA Bo-jun2,ZHANG Hong-sheng1,(1State Key Laboratory of Crop Genetics and Germplasm Enhancement,Nanjing Agricultural University,Nanjing 210095,China,2College of Chemistry and Life Sciences,Zhejiang Normal University,Jinhua 321004,China) |
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| Abstract: | Plant calcineurin B-like(CBL)proteins have been proposed as important Ca2+sensors and specifically interact with CBL-interacting protein kinases(CIPKs)in plant-specific calcium signaling.We identified and isolated 15 CIPKgenes in ja-ponica rice variety Nipponbare based on the predicted sequences of rice CIPKgene family.Gene structure analysis showed that these 15genes were divided into intron-less and intronrich groups,in which OsCIPK3 and OsCIPK24 exhibit alternative splicing.The phylogenetic analysis indi... |
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| Keywords: | rice CIPKfamily gene expression stress bacterial blight |
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