| 土壤接种棉花枯萎病菌的两种方法及适宜接种量 |
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| 引用本文： | 郑鈜爽,冯自力,朱荷琴,李志芳,赵丽红,师勇强.土壤接种棉花枯萎病菌的两种方法及适宜接种量[J].植物保护,2015,41(4):136-138. |
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| 作者姓名： | 郑鈜爽 冯自力 朱荷琴 李志芳 赵丽红 师勇强 |
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| 作者单位： | 河南科技学院,新乡,453003 |
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| 基金项目： | 河南省教育厅科学技术研究重点项目（15A210024）；国家级大学生创新创业训练计划（20121046701703）；新乡市科技发展计划（13SF32） |
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| 摘 要： | 本研究通过两组试验,明确了土壤接种棉花枯萎病菌的两种方法及适宜接种量。枯萎病菌的繁殖采用玉米沙粒培养基。建立苗床病圃或田间接种时,可在种沟内直接将风干的玉米沙粒培养物与土壤混匀,适宜接种量为10g/m。采用土壤拌菌法时,病原菌的风干玉米沙粒培养物的加入量以灭菌的细沙壤土的0.6%~0.8%为宜。采用上述接种方法和接种量,感病品种‘冀棉11’当年病株率可达到80%以上,病情指数可达到50以上。本文提供的接种方法和接种量可用于棉花新品种(系)、资源材料的抗病鉴定及枯萎病的相关研究。
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| 关 键 词： | 基质分散萃取 碳纳米管 菠菜 新烟碱类杀虫剂 高效液相色谱法 多残留分析 |
| 收稿时间： | 2014/5/27 0:00:00 |
| 修稿时间： | 2014/10/6 0:00:00 |
Two methods of soil inoculation of cotton Fusarium pathogen and appropriate inoculum |
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| Zheng Hongshuang,Feng Zili,Zhu Heqin,Li Zhifang,Zhao Lihong,Shi Yongqiang.Two methods of soil inoculation of cotton Fusarium pathogen and appropriate inoculum[J].Plant Protection,2015,41(4):136-138. |
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| Authors: | Zheng Hongshuang Feng Zili Zhu Heqin Li Zhifang Zhao Lihong Shi Yongqiang |
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| Institution: | Henan Institute of Science and Technology, Xinxiang 453003, China |
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| Abstract: | This study established the acetonitrile as dispersion solvent, with DWCNTs-COOH and GCB as dispersion matrix material in dinotefuran, nitenpyram and imidacloprid residue matrix dispersion extraction pretreatment for spinach samples, set up and optimized HPLC of the acetonitrile gradient elution residual sample with quantitative method of external standard. The results showed that the optimal retention time for dinotefuran, nitenpyram and imidacloprid were 6.8, 8.6 and 17.7 min, respectively. They had a good linear relationship with the corresponding chromatographic peak area ranging from 0.025 to 25.00 mg/L in mass concentration. The average recovery of dinotefuran, nitenpyram and imidacloprid was more than 75%; the level of relative standard deviation was less than 10% in spinach. The minimum detectable concentration were 0.091 2 mg/L (dinotefuran), 0.080 8 mg/L (nitenpyram) and 0.062 8 mg/L (imidacloprid) under the conditions of chromatographic method. The results indicated that the residue sample pretreatment methods and sample analysis methods were simple, efficient, economical and reliable, and could meet the requirements for quantitative detection of the three kinds of pesticide residues in spinach. |
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| Keywords: | matrix dispersion extraction carbon nanotubes spinach neonicotinoid insecticides high performance liquid chromatography residual analysis |
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