Analysis of properties of monoclonal antibodies to transmissible gastroenteritis virus using TO-163 strain |
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| Authors: | E Honda H Kanemaru X Y Dong M Ikuma Y Somura I Shibata K Okazaki T Kumagai |
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| Institution: | Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Japan. |
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| Abstract: | Nineteen monoclonal antibodies (MAbs), reactive in enzyme-linked immunosorbent assay (ELISA), with porcine transmissible gastroenteritis (TGE) virus TO-163 were obtained. Of these MAbs, 5 showed neutralizing (NT) activity (x 3,200 to 25,600) against TO-163. One of the MAbs which had NT activity showed hemagglutination inhibition activity (x 5,120) too. 14 hybridomas of polypeptide specificity against TO-163 strain were developed from which 11, 2, and 1 were specific for protein E2, N, and E1, respectively. Immunofluorescence staining patterns in TGE virus-infected cells reacted with MAbs were divided into three groups (types I, II and III). The fluorescence staining of E2 specific MAbs having NT activity were limited to the perinuclear area. All MAbs having NT activity showed the same fluorescence staining pattern. |
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