|
|||||
|
|
Analysis of Indica-Japonica Differentiation in Rice Parents and Derived Lines Using ILP Markers |
|
| Authors: | XU Xu-ming LIANG Kang-jing ZHANG Shou-gang SHANG Wei ZHANG Ying-ying WEI Xin-yu KE Bei |
| Institution: | 1. Key Laboratory of Crop Genetics and Breeding and Comprehensive Utilization,Ministry of Education/Fujian Agriculture and Forestry University,Fuzhoa 350002,P.R.China;Sanming Research Institute of Agriculture Science,Shaxian 365509,P.R.China 2. Key Laboratory of Crop Genetics and Breeding and Comprehensive Utilization,Ministry of Education/Fujian Agriculture and Forestry University,Fuzhoa 350002,P.R.China 3. Sanming Research Institute of Agriculture Science,Shaxian 365509,P.R.China |
| Abstract: | Revealing the indica-japonica differentiation in parents of hybridization between indica and japonica rice and their derived lines can provide theoretical and practical bases for the breeding of practical inter-subspecific hybrid rice. Using subspecies-specific molecular markers ILP (intron length polymorphism) and Cheng's index, the indica-japonica differentiation was analyzed with special materials including 18 indica-japonica hybrid parents and 39 derived lines, which accumulated different wide compatibility and restoring genes by convergent cross method in 21 years spanning four breeding phases. The indica-japonica differentiation was detected on all tested loci in 57 materials. Among the 18 parental lines, 4 were japonica type, 5 japonicaclinous type, 8 indicaclinous type and one indica type. The japonica proportion indexes in indica restorer lines Minghui 63 and 9308 were 12.50 and 33.33%, respectively, while that in japonica restorer line C418 was only 31.25%. Among the 39 derived lines from indica-japonica hybridization, one was japonica type, 11 japonicaclinous type, 20 indicaclinous type and 7 indica type. The japonica proportion index in Minghui 502 was only 10.42%. The results of indica and japonica classification by ILP molecular markers and Cheng's index were relatively consistent. The correlation coefficient between the japonica proportion index and morphology index was 0.794**, while that between the indica proportion index and morphology index was −0.7662**. ILP markers could be used to accurately detect the proportion of indica/japonica content in the genome of a rice variety. The results of indica-japonica differentiation analysis could make reasonable explanation for that the hybrids obtained from indica-japonica type restorer lines had obvious heterosis. This conclusion would provide important guidance in efficient use of beneficial genes of inter-subspecific hybrid rice. |
| Keywords: | rice hybrids between indica and japonica indica-japonica differentiation ILP japonica proportion index |
| 本文献已被 维普 万方数据 ScienceDirect 等数据库收录！ | |