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| 口蹄疫病毒O型OXH0312株灭活疫苗的制备及其检验 | |
| 引用本文： | 王洪巧,赵海源,黄炯,朱刚,郭惠玲,唐磊.口蹄疫病毒O型OXH0312株灭活疫苗的制备及其检验[J].中国兽药杂志,2007,41(3):10-13. |
| 作者姓名： | 王洪巧 赵海源 黄炯 朱刚 郭惠玲 唐磊 |
| 作者单位： | 1. 新疆农业大学,新疆乌鲁木齐,830052;新疆天康畜牧生物技术股份有限公司,新疆乌鲁木齐,830032 2. 新疆天康畜牧生物技术股份有限公司,新疆乌鲁木齐,830032 3. 新疆农业大学,新疆乌鲁木齐,830052;新疆畜牧科学院兽医研究所,新疆乌鲁木齐,830000 |
| 摘 要： | 对口蹄疫O型OXH0312病毒株进行了牛体感染、乳鼠适应传代和细胞适应传代等生物学特性的研究,并对制备的细胞毒配制的疫苗进行了免疫原性测定。结果显示,该毒株从细胞毒13代(BF13)起,病变时间趋于稳定,14代至17代是一个相对平台期,病变时间稳定在11 h之内;用乳鼠测定病毒毒价稳定在107.68/0.2 mL左右,传到21代时,效价开始下降。用15代、16代和17代病毒液制备3批疫苗进行PD50的测定,3批成品苗的PD50分别为3.80、4.20、3.00。说明该毒株具有比较稳定的生物学特性和良好的免疫原性,可以做为生产用后备种毒。 |
| 关 键 词： | 口蹄疫 灭活疫苗 制备 |
| 文章编号： | 1002-1280（2007）03-0010-04 |
| 修稿时间： | 2006-09-04 |
The Manufacturing and Biological Check of Foot and Mouth Disease Virus Type O OXH0312 Inactivated Vaccine |
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| WANG Hong-qiao,ZHAO Hai-yuan,HUANG Jiong,ZHU Gang,GUO Hui-ling,TANG Lei.The Manufacturing and Biological Check of Foot and Mouth Disease Virus Type O OXH0312 Inactivated Vaccine[J].Chinese Journal of Veterinary Drug,2007,41(3):10-13. | |
| Authors: | WANG Hong-qiao ZHAO Hai-yuan HUANG Jiong ZHU Gang GUO Hui-ling TANG Lei |
| Institution: | 1. College of Veterinary of Xinjiang Agriculture University, Wulumuqi, Xinjiang 830052 ; 2. Xinjiang Tiankang Husbandary Biological Technique Company Ltd, Wulumuqi, Xinjiang 830023; 3. Institute of Veterinary Xinjiang Academy Institute of Animal Science, Wulumuqi, Xinjiang 830000;China |
| Abstract: | The biological characteristics of Foot and Mouth disease virus type O OXH0312 was explored through bovine infecting,after mice seed-virus and adapting to BHK21 cell,the vaccine of cell virus was studied in immunogenicity.The results showed that such suitable passages of productive seed-virus as BF13,BF14,BF15,BF16,BF17 were selected due to stable harvest time within 11 h,and mice titration to 107.68/0.2 mL.Then using three virus liquid of ABF15,BBF16,CBF17 as antigen of inactivated vaccine FMD type O were produced accordingly,studying the process of inactivation,which then past examination.It explained that the virus had stabler biological characteristics and well immunogenicity.It may be used as seed-virus in reserve of producing. |
| Keywords: | Foot and Mouth disease type O Inactivated vaccine preparation |
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