| 土壤放线菌分离方法研究 |
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| 引用本文： | 郑雅楠,杨宇,吕国忠,谷祖敏.土壤放线菌分离方法研究[J].安徽农业科学,2006,34(6):1167-1168,1170. |
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| 作者姓名： | 郑雅楠 杨宇 吕国忠 谷祖敏 |
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| 作者单位： | 1. 沈阳农业大学植物保护学院,辽宁,沈阳,110161
2. 大连民族大学生命科学学院,辽宁,大连,116600 |
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| 摘 要： | 研究了土壤放线菌的分离方法,结果表明:培养基中加入150mg/L重铬酸钾,既有利于放线菌的生长,又能抑制真菌和细菌的生长;土样在28℃或室温风干10～20d后,制成10%土壤悬浮液,40～50℃、pH7条件下,加6%酵母膏(或6%蛋白胨)和0.05%SD(S5mmol/L磷酸缓冲液),振荡10～20min,灭菌水稀释成1%后,与含250mg/L重铬酸钾的高氏1号琼脂培养基制成平板,最有利于放线菌的分离。
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| 关 键 词： | 放线菌 分离方法 预处理 抑制剂 |
| 文章编号： | 0517-6611（2006）06-1167-02 |
| 收稿时间： | 2005-12-21 |
| 修稿时间： | 2005-12-21 |
Study on the Method of Actinomycetes Isolation from Soil |
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| ZHENG Ya-nan et ai.Study on the Method of Actinomycetes Isolation from Soil[J].Journal of Anhui Agricultural Sciences,2006,34(6):1167-1168,1170. |
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| Authors: | ZHENG Ya-nan et ai |
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| Institution: | Shenyang Agricultural Uniuersity, Shenyang, Liaoning 110161 |
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| Abstract: | The optimum concentration of the inhibitor potassium dichromate(K2Cr2O7) in the plate medium was studied;meanwhile,the study on pretreatment of soil samples collected from corn field was conducted.The results showed that the suitable method of the selective isolation of actinomycetes was: after the collected soil suspension(10 %) added with 6 % yeast extract and 0.05 % SDS(5 mmol/L phosphate buffer)was stirred at 40 ℃,pH 7 for 20 minutes,or the soil suspension(10 %) added with 6 % peptone and 0.05 % SDS(5 mmol/L phosphate buffer) was stirred at 50 ℃,pH 7 for 10 minutes,then the soil suspension diluted with water to 1 % was inoculated with the method of plate dilution on Gause's starch agar added with 150 mg/L K2Cr2O7 medium for 7 days and these pretreatments were effective on actinomycetes' isolation. |
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| Keywords: | Actinomycetes Isolation Pretreatment Inhibitor |
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