| 仿刺参疣足数量SNP遗传力评估 |
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| 作者姓名： | 倪萍 任强 王静 丁君 常亚青 王扬帆 胡景杰 包振民 |
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| 作者单位： | 中国海洋大学 海洋生物遗传学与育种教育部重点实验室 青岛 266003;大连海洋大学 农业农村部北方海水增养殖重点实验室 大连 116023 |
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| 基金项目： | 国家重点研发计划课题(2018YFD0901601);国家自然科学基金项目(32072976,31900369)共同资助。 |
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| 摘 要： | 随着测序技术的发展,基于单核苷酸多态性(SNP)分子标记的遗传力估计比传统法准确性更高,已被广泛应用于动植物育种中。本研究对不同地理仿刺参(Apostichopusjaponicus)群体的疣足数量进行重测序全基因组水平的SNP遗传力估计,结果显示,次等位基因频率(Minorallele frequency,MAF)>0.05时,在50K SNP基础上均匀抽样,不同SNP密度的仿刺参疣足数量SNP遗传力估计均值范围为(0.566±0.022)~(0.612±0.003);MAF>0.1时,SNP遗传力估计均值范围为(0.586±0.015)~(0.615±0.016),说明50K低密度SNP标记足够捕获数量性状基因座(QTL)大效应和小效应;同时,染色体水平SNP遗传力估计值显示,单个染色体对遗传力的贡献和其长度显著相关,暗示仿刺参疣足数量是一个复杂的数量性状,与该性状相关的基因效应位点散布在各染色体,并由多基因共同作用。本研究结果可为海参低密度SNP芯片的设计开发及海参遗传参数评估提供一定的理论依据。
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| 关 键 词： | 遗传力 REML SNP 仿刺参 疣足数量 |
| 收稿时间： | 2020/12/17 0:00:00 |
| 修稿时间： | 2021/1/18 0:00:00 |
Estimating SNP heritability for papillae number in sea cucumber Apostichopus japonicus |
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| Authors: | NI Ping REN Qiang WANG Jing DING Jun CHANG Yaqing WANG Yangfan HU Jingjie BAO Zhenmin |
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| Institution: | (Ministry of Education Key Laboratory of Marine Genetics and Breeding,College of Marine Life Sciences,Ocean University of China,Qingdao 266003;Key Laboratory of Mariculture&Stock Enhancement in North China’s Sea,Ministry of Agriculture and Rural Affairs,Dalian Ocean University,Dalian 116023) |
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| Abstract: | The estimation of heritability is a fundamental task in the selective breeding of aquatic animals. With the development of sequencing technology, the estimation of heritability based on single nucleotide polymorphism (SNP) markers has been widely used in breeding since it is more accurate than the traditional method. In this study, the genome-wide SNP heritabilities for papillae number in Apostichopus japonicus from different geographic locations were estimated, and the results showed that the estimated mean SNP heritabilities with different SNP densities at MAF (minor allele frequency)>0.05 ranged from 0.566±0.022 to 0.612±0.003, and the estimated mean SNP heritabilities with different SNP densities at MAF>0.1 ranged from 0.586±0.015 to 0.615±0.016 when sampled uniformly on 50K SNPs, indicating that 50K low-density SNP markers are sufficient to capture both large and small effects of the quantitative trait locus. Chromosome-wise SNP heritability estimates showed that the contribution of an individual chromosome was significantly correlated to its length, suggesting that the papillae number is a complex quantitative trait with effect sites scattered across the chromosomes and shared by multiple genes. The results of this study provide a theoretical basis for the design and development of A. japonicus low-density SNP chips and the evaluation of A. japonicus genetic parameters. |
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| Keywords: | Heritability REML SNP Apostichopus japonicus Papillae number |
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