| 玉米叶片早衰突变体les1的生理特性及基因初步定位 |
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| 引用本文： | 司伟娜,高梦,程振,王文龙,程亦峰,路小铎,朱芮,江海洋.玉米叶片早衰突变体les1的生理特性及基因初步定位[J].玉米科学,2021,29(5):50-56. |
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| 作者姓名： | 司伟娜 高梦 程振 王文龙 程亦峰 路小铎 朱芮 江海洋 |
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| 作者单位： | 安徽农业大学生命科学学院, 合肥 230036 |
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| 基金项目： | 国家级大学生创新创业训练计划一般项目（202110364064） |
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| 摘 要： | 通过甲基磺酸乙酯诱变得到玉米叶早衰突变体les1(leaf early senescence 1),突变体les1从7叶期开始叶片由下往上失绿黄化,生长后期叶片黄化干枯面积逐渐增大,且突变体les1地下部分生物量及子粒变小、子粒千粒重显著降低。与叶片黄化表型一致,研究发现,突变体les1叶片中光合色素含量以及净光合速率显著降低,且突变体les1中活性氧及丙二醛大量积累,可能与叶绿素的降解及叶片早衰相关。遗传分析表明,les1的叶片早衰表型由单基因隐性突变所致,进一步通过混池建库和全基因重测序将les1的突变位点初步定位在2号染色体。
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| 关 键 词： | 玉米 突变体 叶片早衰 活性氧 基因初步定位 |
| 收稿时间： | 2021/9/18 0:00:00 |
Physiological Characteristics and Preliminary Gene Mapping of Maize Leaf Premature Senescence Mutant les1 |
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| SI Wei-n,GAO Meng,CHENG Zhen,WANG Wen-long,CHENG Yi-feng,LU Xiao-duo,ZHU Rui,JIANG Hai-yang.Physiological Characteristics and Preliminary Gene Mapping of Maize Leaf Premature Senescence Mutant les1[J].Journal of Maize Sciences,2021,29(5):50-56. |
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| Authors: | SI Wei-n GAO Meng CHENG Zhen WANG Wen-long CHENG Yi-feng LU Xiao-duo ZHU Rui JIANG Hai-yang |
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| Institution: | Anhui Agricultural University, School of Life Sciences, Hefei 230036, China |
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| Abstract: | In the present study, a maize leaf premature senescence mutant les1 was identified. les1 exhibits leaf-yellowing phenotype from seven-leaf stage. During growth, the area of leaf-yellowing was increased gradually. In addition, underground biomass, length of ears, the size of seed and thousand-grain weight were apparently reduced. In accordance with the leaf-yellowing phenotype, the chlorophyll content and the net photosynthetic rate were significantly decreased in les1. ROS and MDA were also significantly accumulated in les1, which may be related to the degradation of chlorophyll and the premature senescence of les1 leaves. Moreover, genetic analysis implied that the leaf premature senescence phenotype of les1 was caused by a recessive mutation in a single locus. The mutate locus of les1 was preliminarily located on chromosome 2 through DNA mixed pool construction and whole-genome widely re-sequencing. |
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| Keywords: | Maize Mutant Leaf premature senescence ROS Preliminary gene mapping |
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