| 绿色荧光蛋白及其在转基因动物研究中的应用 |
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| 引用本文： | 李夏,陈素文,喻达辉.绿色荧光蛋白及其在转基因动物研究中的应用[J].南方水产,2005,1(5):77-80. |
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| 作者姓名： | 李夏 陈素文 喻达辉 |
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| 作者单位： | 中国水产科学研究院南海水产研究所,广东,广州,510300 |
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| 基金项目： | 国家“863”高技术研究发展计划项目(2002AA603022)，广东省科技计划项目(2002B2150101)，广东省自然科学基金(37148) |
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| 摘 要： | 绿色荧光蛋白(greenfluorescentprotein,GFP)是源于水母(Aequoreavictoria)、海笔(Renillareniformis)等海洋无脊椎动物的一种蛋白质,这种蛋白质在体外经适当波长的光激发便可发出绿光,所发出的绿光用普通荧光显微镜或荧光激活细胞分拣器(FACS)均可检测到。GFP基因是目前能在细胞内稳定表达的优良标记基因之一,不需要任何反应底物及其它辅助因子,无种属、组织和位置特异性,对受体细胞基本无毒害,且检测简单,灵敏度高,操作方便,结果可靠,无损于细胞或胚胎的完整性及活力,是一种非常实用的新型报告基因,在细胞生物学和分子生物学领域有着广泛的应用前景。
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| 关 键 词： | GFP 转基因动物 |
| 文章编号： | 1673-2227-(2005)05-0077-04 |
| 修稿时间： | 2005年4月21日 |
Green fluorescence protein and its application in animal transgenic study |
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| LI Xia,CHEN Su-wen,YU Da-hui.Green fluorescence protein and its application in animal transgenic study[J].South China Fisheries Science,2005,1(5):77-80. |
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| Authors: | LI Xia CHEN Su-wen YU Da-hui |
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| Abstract: | Green fluorescent protein (GFP) is a sort of protein produced by jellyfish (Aequorea victoria) and sea pansy (Renilla reniformis) which absorbs blue light and emits green fluorescence without exogenous substrates or co-factors, and the fluorescence can be easily detected with fluorescent microscope or fluorescence-activated cell sorter (FACS).Green fluorescent protein gene is an important new reporter gene that can express in living cells without other external substrate and no specific in species, tissues or cells at present.With features of sensitivity in detection, simplicity in manipulation, and reliability in result, GFP is an important reporter gene of practical. Being an effective selective marker, GFP has a great potential application in fields of cell, developmental and molecular biology. |
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| Keywords: | GFP transgenic animal |
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