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The development of precise genetic stocks in two wheat cultivars and their use in genetic analysis
Authors:V S Arbuzova  T T Efremova  L I Laikova  O I Maystrenko  O M Popova  T A Pshenichnikova
Institution:(1) Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia
Abstract:Summary The results of genetic studies of common wheat that have been conducted in Novosibirsk, Russia, over the past 20 years by a research team are summarized. The research strategy was to develop a collection of aneuploids and substitution lines to be further used for chromosomal localization of genes and in the study of the genetic variability of wheat. On the basis of two cultivars, namely Saratovskaya 29 and Diamant, we have developed 6 sets of aneuploids with a complete set of monosomic lines for each, plus sets of lines ditelosomic and monotelosomic for ldquostandardrdquo arms. Exploiting the monotelosomics, 108 single chromosome intervarietal substitutions, 13 lines with alien substitutions (mono- and disomics) and 11 addition lines have been developed. A collection of lines isogenic for dominant marker genes of morphological characters has also been developed. The genetic collection was used in chromosomal localization of 15 genes, for many of which chromosome arms have been determined. Positively or negatively, the question of allelism within some loci has been answered.
Keywords:aneuploid lines  chromosomal localization of genes  common wheat  substitution lines  triticum aestivum
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