| 中国野生葡萄抗黑痘病基因RAPD标记的克隆、序列分析及辅助育种应用 |
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| 引用本文： | 张剑侠,王跃进,张艳艳,周邦军.中国野生葡萄抗黑痘病基因RAPD标记的克隆、序列分析及辅助育种应用[J].果树学报,2009,26(4). |
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| 作者姓名： | 张剑侠 王跃进 张艳艳 周邦军 |
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| 作者单位： | 陕西省农业分子生物学实验室,西北农林科技大学园艺学院,陕西杨凌,712100;农业部西北园艺植物种质资源利用重点开放实验室 |
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| 摘 要： | 对中国野生葡萄抗黑痘病基因RAPD标记OPS03-1300进行了克隆、测序及序列分析,结果表明该标记实际长度是1354bp,因此更名为OPS03-1354,其GenBank登录号为DQ350885。该标记序列与99条来自欧洲葡萄的EST有同源性,其中与1条来自赤霞珠叶片感染葡萄皮尔斯病病原菌后获得的EST同源性为82%,与2条来自赤霞珠果实在不同发育期获得的EST同源性分别为80%和85%。该序列与1条来自中国野生华东葡萄白河-35-1叶片接种霜霉病病原菌后获得的EST同源性为67%。该序列编码葡萄的一种假定蛋白。此外,应用该标记对中国野生葡萄与欧洲葡萄的种间杂交广西-1×京可晶F1代339株、白河-35-1×佳利酿F2代207株进行了辅助选择。
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| 关 键 词： | 中国野生葡萄 抗黑痘病基因 RAPD标记 克隆 序列分析 标记辅助育种 |
Cloning and sequence analysis of the RAPD marker linked to anthrac-nose-resistance gene in Chinese wild Vitis and its application for marker-assisted breeding |
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| ZHANG Jian-xia,WANG Yue-jin,ZHANG Yan-yan,ZHOU Bang-jun.Cloning and sequence analysis of the RAPD marker linked to anthrac-nose-resistance gene in Chinese wild Vitis and its application for marker-assisted breeding[J].Journal of Fruit Science,2009,26(4). |
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| Authors: | ZHANG Jian-xia WANG Yue-jin ZHANG Yan-yan ZHOU Bang-jun |
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| Institution: | Key Laboratory of Horticultural Plant Germplsm Resources Utilization Northwest of China;Ministry of Agriculture;Shaanxi Province Agri- cultural Molecular Biology Laboratory;College of Horticulture;Northwest A & F University;Yangling;Shaanxi 712100 China |
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| Abstract: | Cloning, sequencing and sequence analysis of the RAPD marker OPS03-1300 linked to anthracnose(Elsinoe ampelina)resistant gene in Chinese wild Vitis were carried out. The result showed that the length of the RAPD marker OPS03-1300 was actually 135 4 bp,so it was renamed as OPS03-1354. Nucleotide sequence data of the RAPD marker had been submitted to the GenBank databases,and the accession number was DQ350885. OPS03-1354 sequence shows identity with 99 EST sequences from Vitis vinifera L.The sequence showed 8... |
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| Keywords: | Chinese wild Vitis Anthracnose-resistance gene RAPD marker Cloning Sequence analysis Marker-assisted breeding Application |
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