| 巴西橡胶树胚性组织长期继代保存及增殖技术 |
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| 引用本文： | 赵辉,崔百明,彭明,王科勇,单国艳,林崇忠.巴西橡胶树胚性组织长期继代保存及增殖技术[J].热带作物学报,2007,28(4):39-43. |
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| 作者姓名： | 赵辉 崔百明 彭明 王科勇 单国艳 林崇忠 |
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| 作者单位： | 1. 华南热带农业大学,海南,儋州,571737
2. 中国热带农业科学院热带生物技术研究所,海口,571101 |
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| 摘 要： | 在橡胶树花药组织培养过程中,利用胚诱导过程后期产生的不同生理阶段的胚性组织进行继代增殖和成胚性研究,试验表明:1)心形胚及前期的胚性组织能继代增殖,多次继代增殖后的组织,经胚诱导过程1~3个月能成倍形成鱼雷胚,大大提高了橡胶花药组培的成胚率;2)胚性愈伤是适合长期继代和增殖保存的最好材料,经2a的继代保存增殖率和成胚率不下降,其最大继代增殖能力还在测试中;3)在继代增殖培养基上,胚性组织按原有形态增殖,通过选取同一生理阶段的胚性组织进行继代,可控制体胚发育过程,促进体胚发育同步进行。
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| 关 键 词： | 巴西橡胶树 花药组培 胚性愈伤 增殖培养 |
| 收稿时间： | 2007-03-05 |
| 修稿时间： | 2007-05-10 |
Proliferation Culture of Somatic Embryonic Tissues of Hevea brasiliensis |
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| Zhao Hui,Cui Baiming,Peng Ming,Wang Keyong,Shan Guoyan,Lin Chongzhong.Proliferation Culture of Somatic Embryonic Tissues of Hevea brasiliensis[J].Chinese Journal of Tropical Crops,2007,28(4):39-43. |
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| Authors: | Zhao Hui Cui Baiming Peng Ming Wang Keyong Shan Guoyan Lin Chongzhong |
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| Institution: | 1. Institute of Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences Haikou Hainan 571101; 2. South China University of Tropical Agriculture Danzhou Hainan 571737 |
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| Abstract: | Proliferation culture was conducted on the different stages of somatic embryonic tissue obtained from the anther culture of Hevea brasiliensis. The different stages of embryonic tissues were maintained under the same proliferation medium. The results showed that the somatic embryonic tissues at or before the stage of heart-shape embryo could be subcultured for proliferation and induced into torpedo embryos after several subcultures, highly increasing the rate of embryo formation during the whole anther culture. The embryonic calli on the first stage of embryo formation were found best for subculture and proliferation, and their growth was healthy after two years of subculture. During the proliferation culture, embryonic tissues proliferated at the same morphological stage as the original, indicating that the development of rubber embryo can be controlled by subculture of a given stage of embryonic tissues. |
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| Keywords: | Hevea brasiliensis anther culture embryonic callus proliferation culture |
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