| 菲和其他芳香族化合物降解菌多酚氧化酶的变化研究 |
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| 引用本文： | 李钠,章骥,闵航,邵爱萍,周红军.菲和其他芳香族化合物降解菌多酚氧化酶的变化研究[J].浙江大学学报(农业与生命科学版),2006,32(5):495-499. |
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| 作者姓名： | 李钠 章骥 闵航 邵爱萍 周红军 |
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| 作者单位： | 浙江大学,生命科学学院,浙江,杭州,310029 |
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| 摘 要： | 以少动鞘氨醇单胞茵(Sphingomonas paucimobilis)ZX4菌株为对象,通过酶活检测和PAGE活性染色,研究了以菲为代表的多环芳烃对细胞内多酚氧化酶的影响.结果表明,经菲诱导后,ZX4菌株多酚氧化酶的活性和组成都发生了明显变化.同时当尼古丁降解茵HF-1菌株、HF-2菌株和烟焦油降解菌MR4菌株受相应污染物诱导后,它们的多酚氧化酶组成也发生明显变化.这些结果表明在降解多环芳烃的过程中多酚氧化酶存在蛋白质的诱导迁移过程,即多酚氧化酶在无多环芳烃的情况下存在于细胞内部,而经多环芳烃诱导后迁移至细胞膜外侧或细胞外.
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| 关 键 词： | 多酚氧化酶 菲 芳香族化合物 尼古丁 烟焦油 |
| 文章编号： | 1008-9209(2006)05-0495-05 |
| 修稿时间： | 2006年3月6日 |
Variation of polyphenoloxidase in phenanthrene-degrading and other aromatic compounds-degrading bacteria |
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| LI Na,ZHANG Ji,MIN Hang,SHAO Ai-ping,ZHOU Hong-jun.Variation of polyphenoloxidase in phenanthrene-degrading and other aromatic compounds-degrading bacteria[J].Journal of Zhejiang University(Agriculture & Life Sciences),2006,32(5):495-499. |
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| Authors: | LI Na ZHANG Ji MIN Hang SHAO Ai-ping ZHOU Hong-jun |
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| Abstract: | Effects of phenanthrene on phenol oxidase of Sphingomonas paucimobilis ZX4 were dealed with by measuring polyphenoloxidase activity and PAGE combined with enzyme activity staining phenol oxidase.When induced by phenanthrene,the activity and constitution of polyphenoloxidase of strain ZX4 changed obviously,so did other bacteria,like nicotine-degrading bacterium HF-1,HF-2 and tobacco tar-degrading bacterium MR4.It shows that at the existence of PAHs,intracellular polyphenoloxidase moves to out side of cell membrane or outside the cell. |
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| Keywords: | polyphenoloxidase phenanthrene aromatic compounds nicotine tobacco tar |
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