Molecular mechanism of P450c17‐II (17, 20‐lyase) regulating gonad development in female Cynoglossus semilaevis |
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| Authors: | Cai‐Fang Chen Hai‐Shen Wen Feng He Ji‐Fang Li Bao‐Xia Qi |
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| Institution: | 1. Fisheries College, Ocean University of China, , Qingdao, China;2. College of Biological and Enviromental Science, Zhejiang Wanli University, , Ningbo, China;3. College of Animal Science and Technology, Inner Mongolia Nationality University, , Tongliao, China |
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| Abstract: | Cytochrome P450c17 (CYP17, 17α‐hydroxylase/17,20‐lyase) is a critical enzyme in the production of androgens and estrogens in vertebrates. A 2102 bp full‐length cDNA of P450c17‐II (CYP17A2) has been isolated from the ovary of half‐smooth tongue sole, Cynoglossus semilaevis which encodes 524 amino acids. The putative P450c17‐II enzyme shares higher sequence identity with those of teleosts than with P450c17‐I of vertebrate. The similarity between the two types of tongue sole P450c17 was 48%.Semi‐quantitative RT‐PCR analysis of spatial expression showed the enzyme was specifically expressed in the ovary and the head kidney. However, temporal expression shows that P450c17‐II can be found in the brain. Furthermore, temporal expression pattern of P450c17‐II in ovary and brain revealed developmental stage‐dependency, and ovary P450c17‐II expressed remarkably throughout the whole reproductive cycle. Otherwise, the expression pattern of P450c17‐II in head kidney indicated negative ovary development‐dependence. In addition, combined with our data on P450c17‐I, T and E2 levels, the results further endorse the critical role of P450c17‐II during shift in steroidogenesis, suggesting that P450c17‐I and ‐II may act together to this physiological process. Based on the present study, we indicate an important role for P450c17‐II during ovarian development. |
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| Keywords: | half‐smooth tongue sole P450c17‐II gene expression steroidogenic shift |
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