| ELISA法与小白鼠生物法检测麻痹性贝类毒素的研究 |
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| 引用本文： | 黄奕雯.ELISA法与小白鼠生物法检测麻痹性贝类毒素的研究[J].福建农业科技,2012(2):47-51. |
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| 作者姓名： | 黄奕雯 |
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| 作者单位： | 福建省海洋环境与渔业资源监测中心,350003 |
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| 摘 要： | 麻痹性贝类毒素(PSP)是当前世界范围内分布最广、危害最大的一种海洋生物毒素。该研究以福建地区贻贝为原料,采用酶联免疫吸附法(ELISA)与传统的小白鼠生物测定法对20份贻贝样品中的麻痹性贝类毒素进行检测,结果表明:两种方法检测麻痹性贝类毒素的结果吻合程度较好,但ELISA法更适于检测PSP含量较小的贝类。通过比较研究,小白鼠生物法操作简单但无法准确定量;而ELISA法快速简便、灵敏度高且检测成本低,对于麻痹性贝类毒素的快速筛选检测具有重要的现实意义。这两种方法结合运用可以确保麻痹性贝类毒素检测的结果更加准确。
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| 关 键 词： | 麻痹性贝类毒素 酶联免疫吸附法 小白鼠生物法 |
Determination of paralytic shellfish poison by means of ELISA and mouse bioassay |
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| HUANG Yi-wen.Determination of paralytic shellfish poison by means of ELISA and mouse bioassay[J].Fujian Agricultural Science and Technology,2012(2):47-51. |
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| Authors: | HUANG Yi-wen |
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| Institution: | HUANG Yi-wen(Monitoring Center of Marine Environment and Fishery Resources of Fuzhou City,Fujian Province 350003) |
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| Abstract: | Paralytic shellfish poison(PSP) is one of marine biotoxins with world-wide distribution and most serious harmness.In this study,20 samples of Mytilus from Fujian were used to determine PSP by ELISA and mouse bioassay.The results of two methods were consistent,but ELISA was more suitable to detect the Mytilus samples with small PSP content.Comparing these two methods,the mouse bioassay was simple,but couldn’t evaluate quantitatively,however,the ELISA assay showed to be simple,sensitive and low cost,which possesses great significance to rapid check out PSP.Combining these two methods could obtain more accurate analyzing results of PSP content. |
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| Keywords: | Paralytic shellfish poison ELISA mouse bioassay |
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