| 蛋氨酸铬对鲤糖代谢相关酶活性及IR、GLUT2和SGLT基因表达的影响 |
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| 引用本文： | 翟胜利,张宝龙,乔秀亭,白东清,程镇燕,孙金辉,尹帅,崔培.蛋氨酸铬对鲤糖代谢相关酶活性及IR、GLUT2和SGLT基因表达的影响[J].大连海洋大学学报,2018(3):316-322. |
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| 作者姓名： | 翟胜利 张宝龙 乔秀亭 白东清 程镇燕 孙金辉 尹帅 崔培 |
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| 作者单位： | 1. 天津农学院 水产学院,天津市水产生态与养殖重点实验室,天津300384;2. 天津晨辉饲料有限公司,天津,301800 |
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| 基金项目： | 天津市高等学校创新团队项目(TD12-5018)，天津市自然科学基金资助项目(14JCQNJC15100)，浙江省重中之重学科开放基金资助项目(xkzsc1501)，农业部热带亚热带水产资源利用与养殖重点实验室开放基金 |
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| 摘 要： | 为探究蛋氨酸铬(CrMet)对鲤Cyprinus carpio糖代谢相关酶活性及糖代谢相关基因表达的影响,以酪蛋白为蛋白源,豆油为脂肪源,配制7组纯化饲料,其中Cr~(3+)水平分别为0(对照)、0.1、0.2、0.4、0.8、1.6、3.2 mg/kg。选取初始体质量为(40.95±4.80)g的鲤,随机分为7组,分别投喂7种饲料,每个组设置3个重复,每个重复放60尾鱼,饲养8周后,检测其肝胰脏糖代谢相关酶活性;禁食48 h后再投喂,再投喂0、3、6、12、24、48 h时检测Cr~(3+)水平为0(对照)、0.8、3.2 mg/kg时鱼肝胰脏IR、GLUT2和肠道SGLT基因的表达量。结果表明:添加0.8 mg/kg Cr~(3+)组的组己糖激酶(HK)、丙酮酸激酶(PK)、磷酸果糖激酶(PFK)和琥珀酸脱氢酶(SDH)活力均显著高于对照组(P0.05),磷酸酵式丙酮酸激酶(PEPCK)活力显著低于对照组(P0.05);而添加Cr~(3+)并未对葡萄糖-6-磷酸脱氢酶(G6PDH)活力产生显著影响(P0.05);投喂24、48 h时,0.8 mg/kg Cr~(3+)组IR mRNA表达量显著高于0、3.2mg/kg Cr~(3+)组(P0.05);投喂3 h时,0.8、3.2 mg/kg Cr~(3+)组GLUT2 mRNA表达量均显著高于对照组(P0.05),12、24、48 h时,0.8 mg/kg Cr~(3+)组GLUT2 mRNA表达量显著高于0、3.2 mg/kg Cr~(3+)组(P0.05);而不同Cr~(3+)添加水平对鲤肠道SGLT mRNA表达量无显著性影响(P0.05)。研究表明,在饲料中添加CrMet能够提高鲤对糖的利用能力,建议Cr~(3+)添加水平为0.8 mg/kg。
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| 关 键 词： | 蛋氨酸铬 鲤 糖代谢相关酶 IR基因 GLUT2基因 SGLT基因 chromium methionine Cyprinus carpio glyco-metabolism-related enzyme IR gene GLUT2 gene SGLT gene |
Effects of chromium methionine on activities of glyco-metabolism-related enzymes and expression of IR,GLUT2 and SGLT genes in common carp Cyprinus carpio |
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| Authors: | ZHAI Sheng-li ZHANG Bao-long QIAO Xiu-ting BAI Dong-qing CHENG Zhen-yan SUN Jin-hui YIN Shuai CUI Pei |
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| Abstract: | A feeding trial was conducted to evaluate the effects of dietary chromium methionine(CrMet) on glyco-metabolism-related enzymes,and expression of IR,GLUT2 and SGLT genes in common carp Cyprinus carpio. Com-mon carp with body weight of(40.95±4.80) g were reared in a rearing system consisting of 21 plastic tanks and fed purified diets containing Cr3+at a dose of 0(control group),0.1,0.2,0.4,0.8,1.6,and 3.2 mg/kg for 8 weeks when activities of glyco-metabolism-related enzymes were tested. IR,and GLUT2 mRNA expression levels in hepa-topancreas and SGLT mRNA expression levels in intestine were analyzed in 0.8 mg/kg group and 3.2 mg/kg group during the refeeding after 48 h fasting. The results showed that the fish fed diets containing 0.8 mg/kg Cr3+had sig-nificantly higher activities of hexokinase(HK),pyruvate kinase(PK),phosphofructose kinase(PFK),and succi-nate dehydrogenase (SDH) and lower phosphopyruvate kinase(PEPCK) activity than the fish in 0 mg/kg group did(P<0.05), without significant difference in G6PDH activity betwween the control and Cr3+supplementation groups(P>0.05). There was significantly higher IR mRNA expression level in 0.8 mg/kg Cr3+group than that in 0 mg/kg group 24 h and 48 h after refeeding(P<0.05), significantly higher GLUT2 mRNA expression level in 0.8 and 3.2 mg/kg Cr3+groups than that in 0 mg/kg group 3 h after refeeding(P<0.05).12,24 and 48 h after refeed-ing,GLUT2 mRNA expression level was significantly higher in 0.8 mg/kg Cr3+group than in 0 and 3.2 mg/kg groups(P<0.05),without significant effect on SGLT mRNA expression level in intestine of common carp fed diets containing differert Cr3+level. The findings indicate that dietary addition of Cr3+leads to significant impact on the carbohydrate utilization of common carp and 0.8 mg/kg is the optimal level of Cr3+in the common carp diet under this experimental condition. |
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