| 湖北部分地区鸭疫里氏杆菌的分离鉴定 |
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| 引用本文： | 艾地云,姚琪,罗玲,邵华斌,杨峻,杨前平,王红琳,温国元,罗青平,张蓉蓉.湖北部分地区鸭疫里氏杆菌的分离鉴定[J].动物医学进展,2011,32(9):46-50. |
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| 作者姓名： | 艾地云 姚琪 罗玲 邵华斌 杨峻 杨前平 王红琳 温国元 罗青平 张蓉蓉 |
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| 作者单位： | 1. 湖北省农业科学院畜牧兽医研究所/动物胚胎工程及分子育种湖北省重点实验室,湖北武汉,430064
2. 武汉理工大学资源与环境工程学院,湖北武汉,430070 |
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| 基金项目： | 湖北省科技攻关计划项目(2007AA201E02);湖北省科技计划研究与开发计划项目(2009BBB003) |
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| 摘 要： | 对湖北省部分地区鸭疫里氏杆菌(RA)进行分离鉴定.应用胰蛋白大豆琼脂(TSA)培养基的分离培养及染色试验、生化试验、PCR鉴定,得到86株RA.染色观察各地RA分离株的细菌形态一致,符合RA特征；生化试验显示86株RA的生理生化特征相似,基本符合RA特征；应用建立的PCR技术均可从这些菌株扩增到RA的特异性基因片段.通...
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| 关 键 词： | 鸭疫里氏杆菌 分离与鉴定 血清型 混合感染 致病性试验 |
Isolation and Identification of Riemerella anatipestifer in Some Areas of Hubei Province |
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| AI Di-yun,YAO Qi,LUO Ling,SHAO Hua-bin,YANG Jun,YANG Qian-ping,WANG Hong-lin,WEN Guo-yuan,LUO Qing-ping,ZHANG Rong-rong.Isolation and Identification of Riemerella anatipestifer in Some Areas of Hubei Province[J].Progress In Veterinary Medicine,2011,32(9):46-50. |
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| Authors: | AI Di-yun YAO Qi LUO Ling SHAO Hua-bin YANG Jun YANG Qian-ping WANG Hong-lin WEN Guo-yuan LUO Qing-ping ZHANG Rong-rong |
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| Institution: | 1(1.Institute of Animal Husbandry and Veterinary Medicine,Hubei Academy of Agricultural Sciences/Hubei Key Laboratory ofAnimal Embryo and Molecular Breeding,Wuhan,Hubei,430064,China;2.School of Natural Resources and Environmental Engineering,Wuhan University of Technology,Wuhan,Hubei,430070,China) |
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| Abstract: | Riemerella anatipestifer(RA) was identified from duck farms in Hubei.The bacteria were identified as RA by the Gram stain test,biochemical reaction and PCR amplifications of the outer membrane gene.Each RA strain originated from different districts had identical morphology,approximate biochemical characteristics and Specific gene fragment of RA was amplified.Slide agglutination test demonstrated that all the RA isolates belong to serotype 1.The RA isolates was isolated on 20 duck farms with different variety ducks in 8 counties of Hubei.The isolation rate of brain(81/86) was significant higher than that of heart blood(53/86) and liver(49/86).Ducklings of 10 day-old to 21 day-old and Cherry valley ducks were more susceptive than that of others to RA infection.A significant age resistance against RA infection was observed during the period of 10 day-old to 42 day-old.28 Escherichia coli were isolated concurrently from the RA positive ducks,and with a 32.56%co-infection rate.Challenge test showed that RA is virulent and even more virulent when co-infected with E.coli.The results showed that RA infection was one of the most common causes for the losses in duck industry. |
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| Keywords: | Riemerella anatipestifer isolation and identification serotype co-infection pathogenicity test |
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