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| 苯酚-硫酸法测定硫酸酯化酵母葡聚糖含量 | |
| 引用本文： | 张健,王成龙,李引乾,丁伯良,秦保亮,王英珍.苯酚-硫酸法测定硫酸酯化酵母葡聚糖含量[J].动物医学进展,2011,32(9):54-57. |
| 作者姓名： | 张健 王成龙 李引乾 丁伯良 秦保亮 王英珍 |
| 作者单位： | 1. 天津市畜牧兽医研究所,天津,300112 2. 天津市畜牧兽医研究所,天津300112;西北农林科技大学动物医学院,陕西杨陵712100 3. 西北农林科技大学动物医学院,陕西杨陵,712100 4. 新乡市畜牧局,河南新乡,453003 |
| 基金项目： | 天津市科技支撑计划重点项目(09ZCGYNC00100) |
| 摘 要： | 参照相关多糖含量的测定方法,以葡萄糖为标准品,应用苯酚-硫酸法对硫酸酯化酵母葡聚糖含量测定的方法进行了研究.该方法在0.010 mg/mL～0.500 mg/mL范围内呈良好的线性关系,线性方程为y=2.025x+0.198 6,R2=0.991 6.另外,还对本方法的重复性、回收率和稳定性等方面进行了试验.结果表明,... |
| 关 键 词： | 硫酸酯化酵母葡聚糖 含量测定 苯酚-硫酸法 |
Determination of Sulfated Yeast Glucan Content Using Phenol-sulfuric Acid Method |
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| ZHANG Jian,WANG Cheng-long,LI Yin-qian,DING Bo-liang,QIN Bao-Liang,WANG Ying-zhen.Determination of Sulfated Yeast Glucan Content Using Phenol-sulfuric Acid Method[J].Progress In Veterinary Medicine,2011,32(9):54-57. | |
| Authors: | ZHANG Jian WANG Cheng-long LI Yin-qian DING Bo-liang QIN Bao-Liang WANG Ying-zhen |
| Institution: | 1 (1.Tianjin Institute of Animal Husbandry and Veterinary Science,Tianjin,300112,China;2.College of Veterinary Medicine,Northwest A&F University,Yangling,Shaanxi,712100,China;3.Animal Husbandry and Veterinary Bureau of Xinxiang,Xinxiang,Henan,453003,China) |
| Abstract: | This study established a method for determination of sulfated yeast glucan content using the phenol-sulfuric acid reaction based on other determinated method of polysaccharide content.This method showed a good linear relationship in the range of 0.010 mg/mL-0.500 mg/mL.Its linear equation is y=2.025x+0.198 6,r2=0.991 6.The repeatability,recovery and stability of this method were detected in this experiment.The results showed that this method can be used for the determination of sulfated yeast glucan content. |
| Keywords: | sulfated yeast glucan content determination phenol-sulfuric acid method |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |