| 小麦avenin-like type-B 基因启动子的分离及表达载体的构建 |
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| 引用本文： | 宋斐,陈泠,孙杨柳,王菲菲,汪越胜,杨广笑,何光源.小麦avenin-like type-B 基因启动子的分离及表达载体的构建[J].广东农业科学,2012,39(4):1-4. |
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| 作者姓名： | 宋斐 陈泠 孙杨柳 王菲菲 汪越胜 杨广笑 何光源 |
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| 作者单位： | 1. 华中科技大学生命科学与技术学院中英HUST-Rres基因工程和基因组学联合实验室/科技部国际科技合作基地(基因工程)/教育部分子生物物理重点实验室,湖北武汉,430074
2. 华中科技大学生命科学与技术学院中英HUST-Rres基因工程和基因组学联合实验室/科技部国际科技合作基地(基因工程)/教育部分子生物物理重点实验室,湖北武汉430074;湖北省农科院粮食作物研究所,湖北武汉430064 |
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| 基金项目： | 国家转基因重大专项(2011ZX08010004-004) |
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| 摘 要： | 根据已知的小麦ALP type-B基因序列,采用反向PCR技术,从小麦品种鄂恩1号(En1)中克隆得到了ALP type-B基因的上游启动子序列1664bp.通过PLACE和plantCARE等数据库对所克隆的启动子进行生物信息学分析,发现该启动子除具备启动子的基本元件如TATA-box、CAAT-box等外,还含有胚乳特异性表达的特有元件如Endosperm motif、GCN4-1ike motif (GLM)、RY motif和G-box等.用ALP type-B启动子置换质粒pBI121上的35S启动子,将其与gus基因连接构建植物表达载体.
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| 关 键 词： | 小麦 胚乳特异性基因 启动子 植物表达载体 |
Isolation of Avenin-like type-B gene promoter from wheat and construction of expression vector |
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| SONG Fei
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CHEN Ling
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SUN Yang-liu
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WANG Fei-fei
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WANG Yue-sheng
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YANG Guang-xiao
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HE Guang-yuan.Isolation of Avenin-like type-B gene promoter from wheat and construction of expression vector[J].Guangdong Agricultural Sciences,2012,39(4):1-4. |
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| Authors: | SONG Fei CHEN Ling SUN Yang-liu WANG Fei-fei WANG Yue-sheng YANG Guang-xiao HE Guang-yuan |
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| Institution: | 1 (1.China-UK HUST-RRes Genetic Engineering and Genomics Joint Laboratory/The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology/Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology,Huazhong University of Science & Technology(HUST),Wuhan 430074,China; 2.Institute of Food Crops,Hubei Academy of Agricultural Sciences,Wuhan 430064,China) |
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| Abstract: | The study successfully cloned a 1 664 bp upsteam sequence of ALP from wheat En1 using Inverse-PCR(IPCR),based on the sequence of ALP type-B gene.Putative fuctional promoter elements were analyzed by the PLACE datebase and PlantCARE datebase.The results showed that the upsteam sequence contained the basic elements such as CAAT-box and TATA-box.Endosperm motif,GCN4-like motif(GLM),RY motif and G-box were also detected as endosperm-specific elements.Expression vector harboring the promoter fused with gus gene was constructed by replacing the 35S promoter in pBI121. |
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| Keywords: | wheat endosperm-specific gene promoter plant expression vector |
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