| 紫外分光光度法测定瑞香狼毒中总香豆素类成分的含量 |
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| 引用本文： | 罗喜荣,龙庆德,杨军,田弋夫.紫外分光光度法测定瑞香狼毒中总香豆素类成分的含量[J].广东农业科学,2012,39(6):98-99. |
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| 作者姓名： | 罗喜荣 龙庆德 杨军 田弋夫 |
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| 作者单位： | 1. 贵阳医学院药学院,贵州贵阳,550004
2. 中国科学院地球化学研究所,贵州贵阳,550002 |
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| 摘 要： | 采用紫外分光光度法测定瑞香狼毒中总香豆素的含量,以补骨脂素为标准品,测定波长为294 nm。结果表明,补骨脂素在4～20μg/mL范围内浓度与吸光度线性关系良好(r=0.9997),精密度RSD为0.06%,稳定性RSD为0.57%,重复性RSD为2.07%,回收率为98.42%～101.34%。该方法准确、快速、重复性好,可用于瑞香狼毒中总香豆素含量的测定。
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| 关 键 词： | 紫外分光光度法 瑞香狼毒 总香豆素 |
Determination of total coumarins in Stellera chamaejasme by UV spectrophotometry |
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| LUO Xi-rong
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LONG Qing-de
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YANG Jun
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TIAN Yi-fu.Determination of total coumarins in Stellera chamaejasme by UV spectrophotometry[J].Guangdong Agricultural Sciences,2012,39(6):98-99. |
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| Authors: | LUO Xi-rong LONG Qing-de YANG Jun TIAN Yi-fu |
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| Institution: | 1.College of Pharmacy,Guiyang Medical College,Guiyang 550004,China; 2.Institute of Geochemistry,Chinese Academy of Sciences,Guiyang 550002,China) |
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| Abstract: | UV spectrophotometry was applied to assay the total coumarins in Stellera chamaejasme.The standard sample was psoralen,detective wavelength was 294 nm.The results showed that the method had good linear relationship within the range of 4～20 μg/mL(r=0.9997),the RSD of precision was 0.06%,the RSD of stability was 0.57%,the RSD of repeatability was 2.07%,the recovery were 98.42%～101.34%.The method is accurate,rapid,reproducible and can be used for measuring the total coumarins in Stellera chamaejasme. |
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| Keywords: | UV spectrophotometry Stellera chamaejasme total coumarins |
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