| 利用农杆菌注射法鉴定番茄黄化曲叶病毒的沉默抑制子 |
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| 引用本文： | 张余洋,耶狄迪亚·咖夫尼,李汉霞.利用农杆菌注射法鉴定番茄黄化曲叶病毒的沉默抑制子[J].中国蔬菜,2012,1(16):32-35. |
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| 作者姓名： | 张余洋 耶狄迪亚·咖夫尼 李汉霞 |
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| 作者单位： | 1.华中农业大学园艺植物生物学教育部重点实验室，湖北武汉 430070,2以色列农业研究组织植物科学研究所，Bet Dagan 50250 |
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| 基金项目： | 国家自然科学基金项目(31171960,31171974);国家“863”计划项目(2012AA100104);国家留学基金项目(2011842261) |
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| 摘 要： | 利用农杆菌注射法鉴定了番茄黄化曲叶病毒的沉默抑制子V2。将分别携带目标基因与GFP表达载体的农杆菌共注射到本氏烟草中，2 d后GFP瞬时高量表达。西瓜褪绿矮化病毒BV1基因表达载体和空载体pBIN分别与GFP共侵染7 d后，GFP表达量由于寄主沉默机制而急剧减弱。V2基因表达载体与GFP表达载体共侵染叶片7 d后，GFP仍保持高水平表达，说明V2作为番茄黄化曲叶病毒沉默抑制子可阻止寄主的沉默机制。农杆菌注射法可快速方便筛选植物病毒沉默抑制子。
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| 关 键 词： | 番茄黄化曲叶病毒 沉默抑制子 农杆菌注射 |
Determination of Silencing Suppressor of Tomato Yellow Leaf Curl Virus by Agroinfiltration |
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| ZHANG Yu-yang
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YEDIDYA Gafni
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LI Han-xia.Determination of Silencing Suppressor of Tomato Yellow Leaf Curl Virus by Agroinfiltration[J].China Vegetables,2012,1(16):32-35. |
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| Authors: | ZHANG Yu-yang YEDIDYA Gafni LI Han-xia |
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| Institution: | 1.Key Laboratory of Horticultural Plant Biology，Ministry of Eduction，Huazhong Agricultural University，Wuhan 430070，Hubei，China|2Institute of Plant Sciences，Agricultural Research Organization of Israel，Bet Dagan 50250，Israel |
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| Abstract: | We described the utilization of agroinfiltration to screen and verify the silencing suppressor，V2，of Tomato yellow leaf curl virus（TYLCV）．The target gene together with the GFP gene was transiently co-expressed in leaves of Nicotiana benthamiana by agroinfiltrations．In all tests of agroinfiltrations，the GFP showed high expression at 2 days post inoculation（dpi），showing the infiltration and expression was successful．On the leaves co-infected with the BV1 gene of Watermelon chlorotic stunt virus or the empty vector pBIN，the GFP fluorescence almost disappeared at 7 dpi due to silencing mechanism of the host plant．The leaves co-infected with V2，showed continuously high expression of GFP，indicating that V2 is the silencing suppressor of TYLCV．Agroinfiltration can be utilized as potent tool for screening the silencing suppressor of plant virus． |
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| Keywords: | Silencing suppressor Agroinfiltration |
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