| 百合花粉管粘附与定向生长相关基因SCA的克隆及基因多样性分析 |
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| 引用本文： | 邬月娟,崔金腾,张克中,贾月慧.百合花粉管粘附与定向生长相关基因SCA的克隆及基因多样性分析[J].分子植物育种,2012(1):12-23. |
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| 作者姓名： | 邬月娟 崔金腾 张克中 贾月慧 |
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| 作者单位： | 北京农学院园林学院 |
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| 基金项目： | 北京市自然科学基金(6122004);北京市教委科技创新平台项目(PXM2009-014207-078529);北京市科技新星项目(2003B013);北京市教育委员会科研水平提高经费共同资助 |
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| 摘 要： | 以亚洲百合‘Tresor’、麝香百合‘White heaven’和东方百合‘Caruso’为试材,首次在百合基因组中扩增到SCA基因全长序列,在亚洲百合和东方百合中分别获得4个SCA基因拷贝,在麝香百合中获得3个SCA基因拷贝,各拷贝之间具有72.97%～99.68%相似性。从百合基因组中克隆到的SCA基因均含有两个外显子和一个内含子,推导编码113～115个氨基酸残基的前体蛋白(其中成熟蛋白均含91个氨基酸残基)。SCA蛋白具有典型的LTP蛋白N端信号肽,保守的8个半胱氨酸残基和2个五肽模序,并且氨基酸序列之间的相似性高于基因序列相似性,为87.91%～98.90%。亚洲百合、麝香百合、东方百合SCA氨基酸序列比对发现在某些氨基酸位点存在着明显种系间差异。在系统进化树中,三个杂种系的11个SCA基因的氨基酸序列与单子叶植物LTP亲缘关系较近,与双子叶植物较远,这与植物分类学中亲缘关系的远近相一致。
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| 关 键 词： | 百合 花粉管粘附 定向生长 SCA 基因多样性 |
Cloning of SCA Gene Related to Pollen Tube Adhesion and Oriented Growth and Analysis of Gene Diversity in Lilium spp. |
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| u Yuejuan,i Jinteng,g Kezhong,ia Yuehui.Cloning of SCA Gene Related to Pollen Tube Adhesion and Oriented Growth and Analysis of Gene Diversity in Lilium spp.[J].Molecular Plant Breeding,2012(1):12-23. |
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| Authors: | u Yuejuan i Jinteng g Kezhong ia Yuehui |
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| Institution: | College of Landscape,Beijing Agricultural College,Beijing,102206 |
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| Abstract: | Stigma/style cysteine-rich adhesin genes(SCA) were cloned from ’Tresor’(Lilium Asiatic Hybrids),’White heaven’(Lilium Longiflorum) and ’Caruso’(Lilium Oriental Hybrids) leaves by PCR approaches.In this research we obtained four gene copies in both of ’Tresor’ and ’Caruse’ genomes and three gene copies in ’White heaven’ genome.The identities of the cloned SCA among the copies were in the ranges from 72.97% to 99.68%.There are two exons and one intron in all of cloned SCA sequence,which could be deduced immature protein with 113 or 115 amino acid residues(encoding mature protein with 91 amino acid residues) SCA protein contains an N-terminal signal peptide of typical LTP protein,eight conserved cysteine residues and two consensus pentapeptide motifs.The identities among the amino acid sequences were in range from 87.91% to 98.90% higher than that of the similarities of gene sequences.Alignment analysis of amino acid sequences of cloned SCAs showed there were some distinct differences of amino acid site existing in the tested three species of lily hybrids.The phylogenetic tree revealed the relationship of the cloned 11 amino acid sequences of SCA were all more close to LTP family of monocotyledons than that ofdicotyledons,which were consistent with the conclusions of kinship in plant taxonomy. |
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| Keywords: | Lily(Lilium spp ) Pollen tube adhesion Oriented growth SCA Gene diversity |
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