| 天门冬多糖对小鼠脾脏淋巴细胞体外增殖的影响 |
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| 引用本文： | 康乐,姚东璧,胡庭俊,李晓栩.天门冬多糖对小鼠脾脏淋巴细胞体外增殖的影响[J].中国畜牧兽医,2010,37(10):15-17. |
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| 作者姓名： | 康乐 姚东璧 胡庭俊 李晓栩 |
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| 作者单位： | （广西大学动物科学技术学院， 南宁 530005） |
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| 基金项目： | 国家自然科学基金，广西"新世纪十百千人才工程"专项资金 |
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| 摘 要： | 本试验旨在研究天门冬多糖对小鼠脾脏淋巴细胞体外增殖的影响。从正常小鼠分离脾脏淋巴细胞体外培养,在细胞培养体系中分别加入终浓度为50、100、200、400 mg/L天门冬多糖或配合刀豆素(5 mg/L)作用24、487、2 h,以MTT法测定细胞的增殖程度。结果表明,天门冬多糖可显著促进体外培养的小鼠脾脏淋巴细胞增殖,并与ConA有协同作用。
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| 关 键 词： | 天门冬 多糖 小鼠 淋巴细胞 增殖 |
The Effect of Asparagus Cochinchinensis Polysaccharide on Proliferation of Splenic Lymphocytes from Mice in vitro |
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| KANG Le,YAO Dong-bi,HU Ting-jun,LI Xiao-xu.The Effect of Asparagus Cochinchinensis Polysaccharide on Proliferation of Splenic Lymphocytes from Mice in vitro[J].China Animal Husbandry & Veterinary Medicine,2010,37(10):15-17. |
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| Authors: | KANG Le YAO Dong-bi HU Ting-jun LI Xiao-xu |
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| Institution: | （College of Animal Science and Technology, Guangxi University, Nanning 530005, China） |
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| Abstract: | To investigate immunomodulatory effect of Asparagus cochinchinensis polysaccharide (ACP)，the murine splenic lymphocytes cells were incubated in RPMI-1640 tissue culture medium supplemented with ConA (final concentration 5 mg/L ). ACP was added at concentrations of 0, 50, 100, 200 or 400 mg /L on 24,48,72 h, respectively. The cell proliferation were observed. The results showed that ACP was able to stimulate proliferation of splenic lymphocytes within 50 to 400 mg/L. |
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| Keywords: | Asparagus cochinchinensis polysaccharide mice lymphocytes proliferation |
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