褐拟谷盗的快速分子鉴定方法 |
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引用本文: | 张呈伟,徐刘平,陆,苗等.褐拟谷盗的快速分子鉴定方法[J].安徽农业科学,2013(24):9924-9926. |
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作者姓名: | 张呈伟 徐刘平 陆 苗等 |
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作者单位: | [1]常州出入境检验检疫局,江苏常州213022 [2]常州市新北区市政园林绿化管理所,江苏常州213000 |
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基金项目: | 江苏出入境检验检疫局科研项目(2010KJ06). |
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摘 要: | 文中应用PCR法和PCR-RFLP法建立了褐拟谷盗的快速分子鉴定方法.结果表明:①PCR法中,采用依据褐拟谷盗COI基因设计的特异性引物进行PCR扩增,其产物经电泳检测证实,能快速准确地鉴定褐拟谷盗;②PCR-RFLP法利用2组简并引物对目标拟谷盗的COI基因进行PCR扩增,并借助HindⅢ限制性内切酶对扩增产物进行酶切后,进行电泳检测,该法也可用于检疫工作中对褐拟谷盗的快速鉴定.
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关 键 词: | 褐拟谷盗 PCR PCR-RFLP 分子鉴定 |
Rapid Molecular Identification of Tribolium destructor |
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Institution: | ZHANG Cheng-wei et ai (Changzhou Entry-Exit Inspection and Quarantine Bureau, Changzhou, Jiangsu 213022) |
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Abstract: | Polyerase chain reaction and polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) analysis were used for discrimination of T. destructor. The results were as follows : (1) The specific primer of PCR can be discriminated T. destructor and oth- er two species of Tribolium. (2) The rapid molecular diagnostic technique for identification of Tribolium( T. destructor; T. confusum; T. castane- urn) was based on PCR-RFLP of the mitochondrial DNA (mtDNA) COI. With'two pairs of primers and one restriction endonucleases (HindI- II), these three species can be discriminated according to the restrictive patterns. This method could be useful in quarantine application. |
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Keywords: | Tribolium destructor PCR PCR RFLP Molecular identification |
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