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雷公藤总甙对马尾松毛虫生物活性的影响
引用本文:周小慧,胡春海,龙绛雪.雷公藤总甙对马尾松毛虫生物活性的影响[J].经济林研究,2005,23(2):17-20.
作者姓名:周小慧  胡春海  龙绛雪
作者单位:中南林学院生命科学与技术学院,湖南,长沙,412004
摘    要:以无水乙醇为溶剂,用连续回流渗漉法从雷公藤生药中提取出粗提物。经乙酸乙酯纯化后得红褐色的浸膏,经检测为总萜内酯(T1)。就其杀虫活性和作用方式进行了较系统的生物测定。结果表明:T1对马尾松毛虫具有强的拒食、麻醉和毒杀作用,毒杀中浓度LC50为1548.50mg/L,拒食中量AFD50为12.25μg/条,麻醉中量ND50为103.98μg/g,无触杀作用;T1不仅抑制马尾松毛虫幼虫的生长发育.还影响其蛹的羽化和卵的孵化及初孵幼虫存活;抑制中浓度EC50为952.65mg/L;在T1浓度10~10000mg/L之间,马尾松毛虫蛹不完全羽化率为43.3%~63.3%。其卵的不能孵化率为12.0%~84.0%,孵化的幼虫存活率为0.0%~95.6%。

关 键 词:雷公藤  杀虫植物  总萜内酯  马尾松毛虫  生物农药  生物活性
文章编号:1003-8981(2005)02-0017-04
收稿时间:2005-04-08
修稿时间:2005年4月8日

Bioactivity of the Terpenoids from Tripterygium wilfordii against Dendrolimus punctatus
ZHOU Xiao-Hui,HU Chun-Hai,LONG Jiang-Xue.Bioactivity of the Terpenoids from Tripterygium wilfordii against Dendrolimus punctatus[J].Economic Forest Researches,2005,23(2):17-20.
Authors:ZHOU Xiao-Hui  HU Chun-Hai  LONG Jiang-Xue
Abstract:Terpenoids were extracted from Tripterygium wilfordii Hook. The results of bioassay showed that the terpenoids had obvious insecticidal activities to Dendrolimus punctatus. The LC50 value of poisoning to the 3rd instarts of the pest insect was 1548. 50 mg/L. The value of median antifeeding dosage (AFD50) of the terpenoids against the 3rd larvae was 12. 25 ug/head. The median narcosis dosage (ND50) was 103. 98 ug/g. The terpenoids did not have contact poisoning to the insect. When the concentration of the terpenoids was between 10 mg/L and 10 000 mg/L, the mortality of the pupae was from 43. 3% to 63. 3%, the rate of unhatched eggs was 12. 0% to 84. 0%, and the rate of living larvae was 0. 0% to 95. 6%.
Keywords:Tripterygium wilfordii Hook  insecticidal plant  terpenoid  Masson-pine caterpillar moths (Dendrolimus punctatus)  bio-pesticide  bioactivity
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