First isolation and molecular characterization of Ehrlichia canis in Spain |
| |
| Authors: | Aguirre Enara Sainz Angel Dunner Susana Amusategui Inmaculada López Lissette Rodríguez-Franco Fernando Luaces Inés Cortés Oscar Tesouro Miguel A |
| |
| Institution: | Departamento de Medicina y Cirugía Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain. |
| |
| Abstract: | This paper reports the first isolation and culture of Ehrlichia canis in Spain from a naturally infected dog using the DH82 cell line. After DNA extraction and PCR amplification, a nearly complete (1412bp) sequence of the 16S rRNA gene of the new E. canis strain was obtained. The GenBank accession number for the nucleotide sequence of this strain is AY394465. This sequence was aligned with the 16S rRNA gene sequences of other Ehrlichia strains accessible in GenBank. The 16S rRNA gene sequence of the E. canis strain reported here showed a high percentage of similarity with the 16S rRNA gene sequence of E. canis from different geographic areas including Japan, Venezuela and Israel. These data confirm the presence of E. canis in Spain. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
