| 柚多聚半乳糖醛酸酶基因家族的鉴定和分析 |
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| 引用本文： | 刘若南,刘林婷,周秋蓉,张蓓,高志键,葛聪,庄木来,李延,王平.柚多聚半乳糖醛酸酶基因家族的鉴定和分析[J].热带作物学报,2022,43(4):666-674. |
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| 作者姓名： | 刘若南 刘林婷 周秋蓉 张蓓 高志键 葛聪 庄木来 李延 王平 |
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| 作者单位： | 1.福建农林大学园艺学院园艺植物遗传育种研究所,福建福州 3500022.福建漳州市平和县农业局,福建平和 3637003.福建农林大学资源与环境学院,福建福州 350002 |
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| 基金项目： | 国家现代农业（柑橘）产业技术体系专项（No.CARS-26）； |
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| 摘 要： | 草莓、桃、苹果等果实在生长后期和贮藏过程中逐渐软化,而柑橘类特别是柚在成熟后期和贮藏过程中出现粒化,严重影响其经济价值.本研究分析和鉴定了柚基因组中多聚半乳糖醛酸酶基因(CgPG)家族成员以及PG酶活的变化,旨在揭示柚果实成熟过程中CgPG表达与汁胞粒化形成的关系.本研究对柚基因组库中CgPG基因家族成员的数量、基因定...
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| 关 键 词： | 柚 多聚半乳糖醛酸酶 汁胞软化 汁胞粒化 |
| 收稿时间： | 2021-09-26 |
Identification and Analysis of polygalacturonasegenes Family in Pomelo |
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| LIU Ruonan,LIU Linting,ZHOU Qiurong,ZHANG Bei,GAO Zhijian,GE Cong,ZHUANG Mulai,LI Yan,WANG Ping.Identification and Analysis of polygalacturonasegenes Family in Pomelo[J].Chinese Journal of Tropical Crops,2022,43(4):666-674. |
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| Authors: | LIU Ruonan LIU Linting ZHOU Qiurong ZHANG Bei GAO Zhijian GE Cong ZHUANG Mulai LI Yan WANG Ping |
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| Institution: | 1. Institute of Genetics and Breeding in Horticultural Plants, College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China2. Pinghe Agriculture Bureau of Fujian Province, Pinghe, Fujian 363700, China3. College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China |
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| Abstract: | Strawberry, peach, apple and some other fruits gradually soften in the late growth period and during storage, while citrus, especially pomelo, granulated in the later ripening and storage, which seriously affects its economic value. In this study, the members of CgPG gene family in the pomelo genome library and the changes of PG enzyme activity were investigated to reveal the relationship between CgPGs expression and juice sacs granulationin the ripening process of pomelo fruit. The number, location and structure of CgPG gene family members in pomelo genome library were analyzed by bioinformatics, and the expression of CgPG gene family members was identified. The degree of granulation and the activities of PG were determined. 26 CgPG genes from pomelo genome were clustered into 6 subgroups (group A-group F) by phylogenetic tree analysis. Except for class E, all exons and introns and motifs were essentially conservative. GDDC domain of CgPG26 in Group E was mutated to GDDA, and SPNTDGI was mutated to APNTDGI respectively. The CgPG genes were mainly distributed on chromosomes 1, 2, 4, 5, 7 and 9. There were 5-6 tandem duplications of genes on both chromosomes 2 and 5. Group A and group B were related to fruit ripening. CgPG5 in group A and CgPG1, CgPG24 in group B were involved in fruit ripening and juice sacs softening, and CgPG13, CgPG12, CgPG26 were involved in pectin degradation and homeostatic regulation in cell wall during pomelo granulation. By expression analysis, CgPG5 in Group A and CgPG1, CgPG24 in Group B might be involved in softening and final decay during the ripening process of the non-granulated fruits, and the significant expression of the genes indicated the juice sacs were decomposed. CgPG5 in Group A and CgPG1, CgPG24 in Group B might be involved in the temporary degradation of pectin during the ripening process of easily granulated fruits. The high expression of the genes made juice sacs softened, which was the optimal time for consumption. The expressions of the genes were highly decreased during the juice sacs granulation. Therefore, CgPG genes expression showed different trends, indicating that CgPG genes might belong to two different regulatory systems in non-granular fruits and easily granular fruits. Among them, CgPG5, CgPG1, CgPG24 belonged to the pectin degradation system of juice cell softening in pomelo at late maturity, while CgPG13, CgPG12, CgPG26 belonged to the cell wall homeostasis regulation system of pomelo granulation. |
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| Keywords: | pomelo polygalacturonase juice sacs softening juice sacs granulation |
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