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栽培奇楠的显微结构研究
引用本文:刘欣怡,王露露,袁靖喆,梅文莉,戴好富,王军.栽培奇楠的显微结构研究[J].热带作物学报,2022,43(5):986-1000.
作者姓名:刘欣怡  王露露  袁靖喆  梅文莉  戴好富  王军
作者单位:1.海南省黎药资源天然产物研究与利用重点实验室/中国热带农业科学院热带生物技术研究所,海南海口 5711012.海南省沉香工程技术研究中心,海南海口 5711013.海南大学林学院,海南海口 570228
基金项目:国家重点研发计划中医药现代化研究重点专项(No.2018YFC1706404);;财政部和农业农村部国家现代农业产业技术体系(No.CARS-21);
摘    要:为探究不同栽培奇楠树所结沉香的显微结构特征,收集15份栽培奇楠树所结沉香样品,通过木材切片和显微观察的方法进行解剖学研究,对其三切面的显微结构特征进行观测、描述、统计、对比和分析,并与普通白木香所结沉香进行对比。结果表明,栽培奇楠树所结沉香样品在显微结构方面有共性特征,其树脂均主要分布于内涵韧皮部、木射线及导管中,树脂丰富且有向木纤维扩散的趋势,在千里香中表现明显,而与之相比,普通白木香所结沉香样品中树脂主要集中于导管中且含量较少,二者差异较大,就树脂富集面积与含量而言,栽培奇楠树所结沉香样品明显优于普通白木香所结沉香样品;此外,部分栽培奇楠所结沉香样品内涵韧皮部与髓心中可见大量草酸钙棱晶,晶体作为树木抵御外界伤害的一种方式,推测其有利于奇楠树受到创伤开始结香后结香面积的扩展与树脂含量的增加;对沉香样品部分特征的定量数据进行统计研究,结果表明部分栽培奇楠树所结沉香样品间有较大差别,如金丝油的管孔密度、直径,内涵韧皮部宽度、密度,射线宽度、间距等与其他样品差异较大,聚类分析结果表明,栽培奇楠树所结沉香金丝油与乌身圆叶较为特殊,位于分支树的根部,千里香、张勺子、透顶绿及凹身木聚为一类,其余9个样品绿油王、‘热科2号'、樱桃叶、糖结、紫棋、蓝宝石、西瓜叶、指天椒及油叶子聚为一类。本研究可为栽培奇楠的树种选育、资源利用、检测鉴定和市场规范化提供依据,尽快完善相关研究才能不断推进沉香产业良性发展。

关 键 词:栽培奇楠  沉香  显微结构  统计分析  
收稿时间:2021-10-26

Microstructure Research of Agarwood from Cultivated Qinan trees
LIU Xinyi,WANG Lulu,YUAN Jingzhe,MEI Wenli,DAI Haofu,WANG Jun.Microstructure Research of Agarwood from Cultivated Qinan trees[J].Chinese Journal of Tropical Crops,2022,43(5):986-1000.
Authors:LIU Xinyi  WANG Lulu  YUAN Jingzhe  MEI Wenli  DAI Haofu  WANG Jun
Institution:1. Key Laboratory for Research and Development of Natural Product from Li Folk Medicine of Hainan Province / Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China2. Hainan Engineering Research Center of Agarwood, Hainan 571101, China3. College of Forestry, Hainan University, Haikou, Hainan 570228, China
Abstract:In order to explore the microstructure characteristics of the agarwood from different cultivated Qinan trees, 15 agarwood samples from cultivated Qinan trees were collected, the anatomical research was carried out by means of wood slicing and microscopic observation. The microstructure characteristics of three sections of the agarwood samples from cultivated Qinan trees were observed, described, counted, compared and analyzed, in the meantime, they were compared with the agarwood from common Aquilaria sinensis. The results indicate that the agarwood samples from cultivated Qinan trees had common characteristics in terms of microstructure, the resin of them were all mainly distributed in the interxylary phloem, wood rays and vessels, what's more, the resin of them were rich and had a tendency to diffuse into the wood fibers, and this characteristic was evident in the agarwood sample from cultivated Qinan Qianlixiang. In contrast, the resin of agarwood from common Aquilaria sinensis sample was mainly concentrated in the vessels and the content was less. There were great differences between two types of agarwood samples. In terms of resin enrichment areas and content, the agarwood samples from cultivated Qinan trees were obviously better than those from common A. sinensis. In addition, a large number of calcium oxalate crystals could be seen in the interxylary phloem and the phloem in the pith of partial agarwood samples from cultivated Qinan trees. Crystals, as a way for trees to resist external damages, which are speculated to be beneficial to the expansion of the resin enrichment areas and the increase of the resin content of the agarwood trees after being wounded and began to form resin. Statistical research on quantitative data of some characteristics indicated that there were great differences between the agarwood samples from cultivated Qinan trees, for example, the density and diameters of pores, the width and density of interxylary phloem as well as rays of Jinsiyou were quite different from other samples. Cluster analysis of quantitative features indicated that ‘Jinsiyou' and ‘Wushenyuanye' were special, located in the root. Qianlixiang, Zhangshaozi, Toudinglü and Aoshenmu are clustered into one clade, and the other nine agarwood samples from cultivated Qinan trees of Lüyouwang, ‘Reke2', Yingtaoye, Tangjie, Ziqi, Lanbaoshi, Xiguaye, Zhitianjiao and Youyezi are clustered into the other one clade. The research could provide basis for varieties breeding, resource utilization, testing, identification, and market standardization of the cultivated Qinan trees. Only by perfecting relevant research as soon as possible can we continue to promote the sound development of the agarwood industry.
Keywords:cultivated Qinan trees  agarwood  microstructure  statistical analysis  
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