| 粉垄耕作对甘蔗土壤微生物群落的影响 |
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| 引用本文： | 刘洪,韦本辉,党柯柯,彭俊伟,李建刚,董元华.粉垄耕作对甘蔗土壤微生物群落的影响[J].热带作物学报,2022,43(3):597-605. |
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| 作者姓名： | 刘洪 韦本辉 党柯柯 彭俊伟 李建刚 董元华 |
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| 作者单位： | 1.中国科学院土壤环境与污染修复重点实验室/南京土壤研究所,江苏南京 2100082.中国科学院大学,北京 1000493.广西壮族自治区农业科学院经济作物研究所,广西南宁 530007 |
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| 基金项目： | 国家重点研发计划项目（No.2017YFD0200604）； |
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| 摘 要： | 粉垄耕作是一种新型的深耕深松的耕作技术,与常规耕作方式不同,粉垄耕作能增加耕层土壤深度,从而更好地改善土壤结构.为揭示不同耕作方式对甘蔗种植土壤细菌和真菌群落的影响,本研究以粉垄耕作和常规耕作的土壤为研究对象,通过16S rRNA和18S rRNA高通量测序技术分析不同生态位(非根际、根际和根表)土壤的细菌和真菌群落的...
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| 关 键 词： | 土壤理化性质 微生物群落 粉垄 高通量测序 耕作方式 |
| 收稿时间： | 2021-10-27 |
Effect of Deep Vertical Rotary Tillage on Microbial Community in Sugar Cane Soil |
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| LIU Hong,WEI Benhui,DANG Keke,PENG Junwei,LI Jiangang,DONG Yuanhua.Effect of Deep Vertical Rotary Tillage on Microbial Community in Sugar Cane Soil[J].Chinese Journal of Tropical Crops,2022,43(3):597-605. |
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| Authors: | LIU Hong WEI Benhui DANG Keke PENG Junwei LI Jiangang DONG Yuanhua |
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| Institution: | 1. Key Laboratory of Soil Environment and Pollution Remediation, Chinese Academy of Sciences / Institute of Soil Science, Chinese Academy of Sciences, Nanjing, Jiangsu 210008, China2. University of Chinese Academy of Sciences, Beijing 100049, China3. Cash Crops Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi 530007, China |
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| Abstract: | Deep vertically rotary tillage is a new type of deep ploughing and deep loosening farming technology. Different from conventional farming methods, deep vertically rotary tillage could increase the depth of the cultivated layer soil, thereby better improving the soil structure. In order to reveal the influence of different farming methods on the bacterial and fungal communities of sugarcane planting soil, this study took deep vertically rotary tillage farming and conventional farming treated soil as the research object, and analyzed different ecological niches through 16S rRNA and 18S rRNA high-throughput sequencing technologies (bulk soil, rhizosphere, and rhizoplane) soil bacterial and fungal community composition, structure and diversity changes, combined with soil chemical properties to carry out correlation analysis. The results showed that compared with conventional tillage treatments, the proportion of small and medium aggregates (<1 mm) increased after deep vertically rotary tillage treatment and the proportion of larger aggregates (>1 mm) was lower than that of the conventional tillage treatment. The content of available phosphorus and soil organic matter in the soil treated by deep vertically rotary tillage increased significantly, while the content of total nitrogen and total phosphorus decreased. The relative abundance of phyla Firmicutes and Chloroflexi significantly increased after deep vertically rotary tillage treatment, and the alpha diversity of bacterial and fungal communities after deep vertically rotary tillage treatment increased obviously, while there was no significance observed in the microbial alpha diversity among different niches of soils. In terms of the differences in microbial beta diversity, compared with the conventional tillage treatment, the soil microbial community structure in the deep vertically rotary tillage treatment altered significantly. At the same time, the bacterial community structures varied from different niches, while no obvious difference was observed in the fungal community structure among different niches. Combined with the correlation analysis of environmental factors, the changes in total nitrogen, available phosphorus, and organic matter content could significantly affect the soil microbial characteristics, and the available phosphorus and soil organic matter were the main factors affecting the composition of soil microbial communities. In summary, the composition of sugarcane soil nutrients and aggregates altered under different soil tillage treatments, and there were differences in the structure and diversity of soil bacterial and fungal communities. The diversity and structure of microbial communities under different tillage methods were affected by soil available phosphorus and organic matter. These research results can provide a theoretical basis for analyzing the response mechanism of sugarcane soil microbial communities to different tillage methods. |
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| Keywords: | soil chemical properties microbial community deep vertically rotary tillage high-throughput sequencing tillage methods |
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