| 高耐镉细菌Burkholderia sp.DF3-1对镉的吸附特性及机理 |
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| 引用本文： | 李卓阳,曹苗苗,周登博,王尉,戚春林,谢江辉.高耐镉细菌Burkholderia sp.DF3-1对镉的吸附特性及机理[J].热带作物学报,2022,43(3):589-596. |
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| 作者姓名： | 李卓阳 曹苗苗 周登博 王尉 戚春林 谢江辉 |
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| 作者单位： | 1.海南大学生态与环境学院,海南海口 5702282.中国热带农业科学院热带生物技术研究所,海南海口 571101 |
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| 基金项目： | 国家自然科学基金面上项目（No.32072504）；;国家现代农业产业技术体系建设项目（No.CARS-31-06）； |
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| 摘 要： | 基于前期从重金属矿区污染土壤中分离和鉴定的一株伯克霍尔德氏菌Burkholderia sp.DF3-1,分析其对环境中镉离子(Cd2+)的吸附特性.通过测定不同初始浓度、pH及培养时间下菌株对镉离子的去除效率,并利用扫描电镜和透射电镜观察含镉环境对菌体细胞内外形态的影响,以及红外光谱和质粒消除实验测定菌体表面基团和初步...
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| 关 键 词： | 镉 Burkholderia sp.DF3-1 吸附 形态结构 机制 |
| 收稿时间： | 2021-09-10 |
Adsorption Characteristics and Mechanism of High Cadmium-tolerant Bacteria Burkholderia sp. DF3-1 to Cadmium |
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| LI Zhuoyang,CAO Miaomiao,ZHOU Dengbo,WANG Wei,QI Chunlin,XIE Jianghui.Adsorption Characteristics and Mechanism of High Cadmium-tolerant Bacteria Burkholderia sp. DF3-1 to Cadmium[J].Chinese Journal of Tropical Crops,2022,43(3):589-596. |
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| Authors: | LI Zhuoyang CAO Miaomiao ZHOU Dengbo WANG Wei QI Chunlin XIE Jianghui |
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| Institution: | 1. College of Ecology and Environment, Hainan University, Haikou, Hainan 570228, China2. Institute of Tropical Bioscience and Biotechnology, China Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China |
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| Abstract: | A Burkholderia sp. DF3-1 strain was isolated and identified previously from contaminated soil in heavy metal mining areas. The strain was preserved in the laboratory of Research Group for Banana Industry Technology. The stain has a strong ability to adsorb cadmium ion. Its adsorption characteristics to cadmium ion (Cd2+) of the environment were analyzed further in this study. The adsorption characteristics for environmental cadmium ion (Cd2+) were further analyzed by multiple ways. The removal efficiency of cadmium ion by the strain was measured under different initial concentrations, pH and culture time. Effects of cadmium on the internal and external morphology of bacterial cells were detected using a scanning electron microscopy (SEM) and a transmission electron microscopy (TEM). The infrared spectroscopy (Fourier transform infrared spectroscopy, FTIR) was used to determine the changes of different groups on bacterial surface. Location of cadmium tolerance genes was also predicted by plasmid elimination. The results showed that the growth of this strain was not almost affected under the initial Cd2+ concentration less than10 mg/L. On the contrary, the initial concentrations of 50 mg/L and 100 mg/L inhibited the growth of Burkholderia sp. DF3-1. Maximum biomass was achieved within 24 hours of culture and decreased in the following tested time points. By analyzing the effects of different pH on the removal efficiency of Burkholderia sp. DF3-1, it was found that the highest removal efficiency was observed at pH 5. The highest removal efficiency was 83.64%. The results of SEM showed that Cd 2+ treatment caused the surface of the bacterial cells to be rough, deformed and shrunken. The results of TME demonstrated that Cd2+ thickened the cell wall and dissolved the bacterial cell membrane. The boundary between cell membrane and cytoplasm was not obvious. The electron microscopy showed that cadmium had a toxic effect on cells. In addition, the results of FTIR showed that the -CH2-, amide I, -NO2, -COOH, -C-OH and -CO- groups were involved in the adsorption process of Cd2+ on the bacterial surface. After using SDS and sodium benzoate to eliminate plasmids, there was little difference in cadmium resistance of Burkholderia sp. DF3-1, so it was preliminarily speculated that the cadmium resistance gene of Burkholderia sp. DF3-1 was located in genomic DNA and not in the plasmid. Therefore, the adsorption of cadmium ions by Burkholderia sp. DF3-1 occurs in both intracellular accumulation and extracellular adsorption. The initial concentration and pH have a greater impact on its adsorption capacity. The above research results would provide an important reference for the later soil bioremediation. |
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| Keywords: | cadmium Burkholderia sp DF3-1 adsorption morphological structure mechanism |
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