| 菲律宾火焰兰(Renanthera philippinensis)组织培养衍生群体表型性状评价及遗传多样性分析 |
| |
| 引用本文： | 邬静,林针懿,张叶,刘乐,郭璁,叶蓓蕾,黎维诗,凌鹏.菲律宾火焰兰(Renanthera philippinensis)组织培养衍生群体表型性状评价及遗传多样性分析[J].热带作物学报,2022,43(3):499-508. |
| |
| 作者姓名： | 邬静 林针懿 张叶 刘乐 郭璁 叶蓓蕾 黎维诗 凌鹏 |
| |
| 作者单位： | 1.海南大学热带特色林木花卉遗传与种质创新教育部重点实验室,海南海口 5702282.海南博大兰花科技有限公司,海南海口 570311 |
| |
| 基金项目： | 海口市重点科技计划项目(2020-039) |
| |
| 摘 要： | 对204株菲律宾火焰兰(Renanthera philippinensis)组织培养衍生群体的26个主要表型性状和遗传多样性水平进行调查与分析,并利用ISSR分子标记对该群体的遗传多样性进行验证.结果表明:7个外观株型性状的遗传多样性指数为1.85,19个花部数量性状的遗传多样性指数为1.87.通过主成分分析,提取了7...
|
| 关 键 词： | 菲律宾火焰兰 表型性状 组织培养衍生群体 ISSR 遗传多样性 |
| 收稿时间： | 2021-08-09 |
Phenotypic Identification and Genetic Diversity Analysis of Tissue Culture Derived Population of Renanthera philippinensis |
| |
| WU Jing,LIN Zhenyi,ZHANG Ye,LIU Le,GUO Cong,YE Beilei,LI Weishi,LING Peng.Phenotypic Identification and Genetic Diversity Analysis of Tissue Culture Derived Population of Renanthera philippinensis[J].Chinese Journal of Tropical Crops,2022,43(3):499-508. |
| |
| Authors: | WU Jing LIN Zhenyi ZHANG Ye LIU Le GUO Cong YE Beilei LI Weishi LING Peng |
| |
| Institution: | 1. Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants, Ministry of Education, Hainan University, Haikou, Hainan 570228, China2. Hainan Boda Orchid Technology Co., Ltd., Haikou, Hainan 570311, China |
| |
| Abstract: | 26 phenotypic traits of 204 plants of Renanthera philippinensis population derived from tissue culture were studied to understand the genetic diversity levels of the tissue culture population. Molecular makers such as ISSR markers were used in the study. The results showed that the genetic diversity index of 7 morphological traits was 1.85, and 19 quantitative traits of flower part diversity index was 1.87. The principal component analysis showed that there were 7 principal components of a cumulative contribution rate 67.887%. The main traits leading to the differences among the individuals were whether all flower branches were on the same surface or on the different faces, the thickness of petal, the length of lateral branch, the length of column bearing stamens and pistils, the width of middle calyx, thenumber of lateral branch, and the number of leaf. Cluster analysis on the data of phenotypic traits revealed that the 204 plants were divided into different four groups. Group I consisted of 78 plants, which had much more leaves. Group II contained 11 plants, which had more branches and flowers. Group III had 31 plants, which had shorter first inflorescence branch and shorter leaf length. Group IV contained 70 plants, which had much shorter and narrower style. Group V contained 14 plantswhich had larger pedicel diameter and wider calyx. ISSR markers were used to confirm the result from cluster analysis, and the results showed that 17 polymorphic bands were amplified by 2 pairs of ISSR primers on 24 plants, on average there were 8.5 polymorphic bands amplified by each pair of primers. The research results would provide a new way to seek genetic variation and an important genetic resource in R. philippinensis variety improvements. |
| |
| Keywords: | Renanthera philippinensis phenotypic trait tissue culture derivation ISSR genetic diversity |
|
| 点击此处可从《热带作物学报》浏览原始摘要信息 |
| 点击此处可从《热带作物学报》下载免费的PDF全文 |
|
