| 不同品系球等鞭金藻在生长及脂肪酸组成上的种内差异 |
| |
| 引用本文： | 丛超,胡冬雪,孙春晓,乔洪金,王际英,张利民.不同品系球等鞭金藻在生长及脂肪酸组成上的种内差异[J].中国水产科学,2017,24(4):774-782. |
| |
| 作者姓名： | 丛超 胡冬雪 孙春晓 乔洪金 王际英 张利民 |
| |
| 作者单位： | 1. 上海海洋大学 水产与生命科学学院, 上海 201306;山东省海洋资源与环境研究院, 山东省海洋生态修复重点实验室, 山东 烟台 264006;2. 山东省海洋资源与环境研究院, 山东省海洋生态修复重点实验室, 山东 烟台 264006 |
| |
| 基金项目： | 国家自然科学基金项目(31201973)，山东省优秀中青年科学家科研奖励基金项目(BS2013HZ018)，国家海洋生物产业-水生动物营养与饲料研发创新示范平台项目(201601003) |
| |
| 摘 要： | 球等鞭金藻作为一种优良的单细胞生物饵料具有选育价值。目前我国水产养殖上使用的大多数球等鞭金藻品系都来源于同一株藻株Isochrysis galbana OA-3011,经过多年的地理隔离形成了不同的品系,为了揭示地理隔离对球等鞭金藻表型性状的影响,从而为球等鞭金藻的选育工作提供依据,我们对5个品系的球等鞭金藻(I.galbana FACHB-861,OA3011-QD,OA3011-LK,OA3011-AY和OA3011-LZ)的种属关系、生长和脂肪酸组成进行了比较分析。实验在相同条件下对5个品系球等鞭金藻培养6 d,提取基因组DNA并PCR扩增测定18S rDNA基因序列,每天定时记录各品系的OD_(660),绘制生长曲线,在进入稳定期后收获并进行生物量的测定及脂肪酸的气相色谱分析。18S rDNA基因序列的进化分析表明,OA3011-LK、OA3011-AY和OA3011-LZ具有一致的序列,而OA3011-QD因1个碱基的差异与其他OA3011出现了分离,FACHB-861与OA3011品系亲缘关系较远;对生长和脂肪酸进行对比分析后,结果显示,5个品系的球等鞭金藻在不同的时间达到稳定期,其中FACHB-861表现出了与其他品系明显不同的生长模式。FACHB-861和OA-3011品系的生物量具有显著差异,OA3011-LK、OA3011-AY和OA3011-LZ之间也有显著差异(P0.05),但特异生长率各品系间无显著差异(P0.05)。FACHB-861和OA3011-LZ的总脂肪酸(TFA)含量显著低于OA3011-LK、OA3011-QD和OA3011-AY(P0.05)。FACHB-861的脂肪酸各成分与OA-3011各品系明显不同。然而,在OA-3011各品系间脂肪酸组成也有明显差异,这表明球等鞭金藻存在广泛和频繁的数量性状表型差异。总之,地理隔离造成的这些差异为球等鞭金藻的选育提供了可行性。根据生长速度或DHA、EPA、多不饱和脂肪酸含量等筛选标准初步分析显示,可对OA3011-LK,OA3011-QD进行进一步的选育。
|
| 关 键 词： | 球等鞭金藻 品系 生长 脂肪酸 遗传差异 |
| 修稿时间： | 2017/7/21 0:00:00 |
Intraspecific variability in growth and fatty acid composition among five strains of Isochrysis galbana |
| |
| CONG Chao,HU Dongxue,SUN Chunxiao,QIAO Hongjin,WANG Jiying,ZHANG Limin.Intraspecific variability in growth and fatty acid composition among five strains of Isochrysis galbana[J].Journal of Fishery Sciences of China,2017,24(4):774-782. |
| |
| Authors: | CONG Chao HU Dongxue SUN Chunxiao QIAO Hongjin WANG Jiying ZHANG Limin |
| |
| Institution: | 1. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China;2. Shandong Provincial Key Laboratory of Restoration for Marine Ecology, Shandong Marine Resource and Environ-ment Research Institute, Yantai 264006, China |
| |
| Abstract: | Isochrysis galbana is an excellent unicellular bait for the larvae of many aquaculture animals. Thus, screening for I. galbana strains with rapid growth rate and high content of docosahexaenoic acid (DHA) and ei-cosapentaenoic acid (EPA) is very important for the breeding of aquaculture animals. However, most of the I. galbana strains in China have been derived from a common ancestor called I. galbana OA-3011, and different strains of I. galbana have been developed after a long duration of geographical isolation. Whether the traits of these strains have changed or not remains unknown. In order to evaluate the status of the phenotypic traits of dif-ferent geographic isolates for future selective breeding, comparative analysis of five strains of I. galbana (FACHB-861, OA3011-QD, OA3011-LK, OA3011-AY, and OA3011-LZ) with regard to their taxonomy, growth, and fatty acid composition was conducted. Different strains were cultured under identical conditions for six days until they reached a stationary phase. Genomic DNA was extracted for polymerase chain reaction sequencing of the 18S rDNA gene. The OD660 was recorded daily for plotting a growth curve. When the cultures reached statio-nary phase, they were harvested by high-speed centrifugation for biomass determination and fatty acid analysis by using gas chromatography. Phylogenetic analysis of the 18S rDNA gene suggested that FACHB-861 was distantly related with the OA-3011 strains. OA3011-LK, OA3011-AY, and OA3011-LZ had identical 18S rDNA sequences, but the 18S rDNA sequence of OA3011-QD differed from that of the other OA-3011 strains at one position, re-sulting in a single base substitution. The growth curve showed that the five strains reached stationary phase at dif-ferent times. FACHB-861 showed a distinct pattern of growth, unlike the other four OA-3011 strains. The final biomass concentrations were significantly different between I. galbana FACHB-861 and OA-3011 strains as well as among OA3011-LK, OA3011-AY, and OA3011-LZ (P<0.05), whereas the growth rates were not significantly different among the strains (P>0.05). The total fatty acid content of FACHB-861 and OA3011-LZ were signifi-cantly lower than that of OA3011-LK, OA3011-QD, and OA3011-AY (P<0.05). The fatty acid profile of FACHB-861 was remarkably different from that of the OA-3011 strains. However, a conspicuous variation in fatty acid compositions was also observed among OA-3011 strains, indicating a wide and frequent quantitative pheno-typic variation among the strains of I. galbana. These variations resulting from geographic isolation might provide a feasible method for the selective breeding of I. galbana strains. According to the screening criteria such as growth rate or DHA, EPA, and polyunsaturated fatty acid content, our preliminary analysis showed that OA3011-LK, which has the highest growth rate, and OA3011-QD, which contains the highest amount of DHA, are promising candidates for further screening. |
| |
| Keywords: | Isochrysis galbana strain growth fatty acid genetic variation |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国水产科学》浏览原始摘要信息 |
| 点击此处可从《中国水产科学》下载免费的PDF全文 |
|
