| 类M蛋白亚单位疫苗的佐剂筛选 |
| |
| 引用本文： | 苏良科,陆承平.类M蛋白亚单位疫苗的佐剂筛选[J].中国兽药杂志,2004,38(1):15-18. |
| |
| 作者姓名： | 苏良科 陆承平 |
| |
| 作者单位： | 南京农业大学农业部动物疫病诊断与免疫重点开放实验室,江苏南京,210095 |
| |
| 摘 要： | 将铝胶、ISA206和CpG三种佐剂分别与热盐酸提取的马链球菌兽疫亚种(Streptococcus equi subsp. zooepidemicus)ATCC35246株类M蛋白配合,制成疫苗接种20日龄的小鼠,并设不加佐剂的类M蛋白亚单位疫苗免疫对照组.二次免疫后用ATCC35246株活菌攻击,铝胶、ISA206、CpG和对照组的保护率分别为75%(12/16)、81.25%(13/16)、68.75%(11/16)和56.25%(9/16).试验结果表明ISA206的免疫增强作用高于其他两种佐剂,可望与类M蛋白亚单位疫苗配合,用于预防猪链球菌病.
|
| 关 键 词： | 马链球菌兽疫亚种 类M蛋白 佐剂 筛选 |
| 文章编号： | 1002-1280(2004)01-0015-04 |
| 修稿时间： | 2003年7月7日 |
Selection of the adjuvants for preparing subunit vaccine of M-like protein |
| |
| SU Liangke,LU Chengping.Selection of the adjuvants for preparing subunit vaccine of M-like protein[J].Chinese Journal of Veterinary Drug,2004,38(1):15-18. |
| |
| Authors: | SU Liangke LU Chengping |
| |
| Abstract: | Three adjuvants, aluminium hydroxide, ISA206 and bacterial DNA(CpG), were separately injected into 20-day-old mice mixed with M-like protein extracted from Streptococcus equi subsp. zooepidemicusstrain ATCC35246 by hot-acid method. Mice injected with subunit vaccine of M-like protein without any adjuvant were set as control group. After two immunizations, mice were challenged by S. zooepidemicus strain ATCC35246. The mice of four test groups had the protective rates of 75%(12/16), 81.25%(13/16), 68.75%(1/16) and (56.25%)(9/16) respectively. The results showed that immune enhancement of ISA206 adjuvant was higher than that of aluminium hydroxide and CpG. It suggested that ISA206 could be used as a immunoadjuvant for the subunit vaccine of M-like protein. |
| |
| Keywords: | Streptococcus equi subsp zooepidemicus M-like protein subunit vaccine |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
